常用缓冲溶液的配制和PH计校正溶液配置方法

1、常用缓冲溶液的配制方法1 甘氨酸盐酸缓冲液（0.05mol/L）X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀释至200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量 = 75.07，0.2 mol/L甘氨酸溶液含15.01克/升。 2邻苯二甲酸盐酸缓冲液（0.05 mol/L）X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCl，再加水稀释到20毫升pH(20XYpH(20XY2.22.42.62.83.05555

2、54.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量 = 204.23，0.2 mol/L邻苯二甲酸氢溶液含40.85克/升 3磷酸氢二钠柠檬酸缓冲液pH0.2mol/L Na2HPO4（毫升）0.1mol/L柠檬酸（毫升）pH0.2mol/L Na2HPO4（毫升）0.1mol/L柠檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8

3、610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量 = 14.98，0.2 mol/L溶液为28.40克/升。

4、Na2HPO4·2H2O分子量 = 178.05，0.2 mol/L溶液含35.01克/升。C4H2O7·H2O分子量 = 210.14，0.1 mol/L溶液为21.01克/升。 4柠檬酸氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7·H2O氢氧化钠(克NaOH 97%盐酸（毫升）HCl（浓）最终体积（升）2.23.13.34.35.35.86.50.200.200.200.200.350.450.38210210210210245285266848383831441861561601161064568105126101010

5、10101010     使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50% 氢氧化钠溶液或浓盐酸调节，冰箱保存。5柠檬酸柠檬酸钠缓冲液（0.1 mol/L）pH0.1   mol/L柠檬酸（毫升）0.1   mol/L柠檬酸钠（毫升）pH0.1   mol/L柠檬酸（毫升）0.1   mol/L柠檬酸钠（毫升）3.03.23.43.63.84.04.24.44.64.818.617.216.014.914.013.112.311.410.39.21.

6、42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.6柠檬酸C6H8O7·H2O：分子量210.14，0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O7·2H2O：分子量294.12，0.1 mol/L溶液为29.41克/毫升。 6乙酸乙酸钠缓冲液（0.2 mol/L） pH(180.2   mol/LNaAc(毫升0.3 

7、  mol/LHAc(毫升 pH(180.2   mol/LNaAc(毫升0.3   mol/LHAc(毫升2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac·3H2O分子量 = 136.09，0.2 mol/L溶液为27.22克/升。 7磷酸盐缓冲液（1）磷酸氢二钠磷酸二氢钠缓冲液（0.2）&

8、#160;pH0.2   mol/LNa2HPO4(毫升0.3   mol/LNaH2PO4(毫升 pH0.2   mol/L Na2HPO4(毫升0.3   mol/LNaH2PO4(毫升5.85.96.06.16.26.36.46.56.66.76.86.98.010.012.315.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.556.551.045.07.07.17.27.37.47.57.67.77.87.

9、98.061.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.016.013.010.58.57.05.3Na2HPO4·2H2O分子量 = 178.05，0.2 mol/L溶液为85.61克/升。Na2HPO4·12H2O分子量 = 358.22，0.2 mol/L溶液为71.64克/升。Na2HPO4·2H2O分子量 = 156.03，0.2 mol/L溶液为31.21克/升。（2）磷酸氢二钠磷酸二氢钾缓冲液（1/15 mol/L）pHmol/L/15Na2HPO4(毫升mol/L/15K

10、H2PO4(毫升pHmol/L/15Na2HPO4(毫升mol/L/15KH2PO4(毫升4.925.295.916.246.476.646.816.980.100.501.002.003.004.005.006.009.909.509.008.007.006.005.004.007.177.387.738.048.348.678.187.008.009.009.509.759.9010.003.002.001.000.500.250.100Na2HPO4·2H2O分子量 = 178.05，1/15M溶液为11.876克/升。KH2PO4分子量 = 136.09，1/15M溶液为9.

11、078克/升。 8磷酸二氢钾氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升pH(20X (毫升Y（毫升）pH(20X (毫升Y（毫升）5.86.06.26.46.66.85555550.3720.5700.8601.2601.7802.3657.07.27.47.67.88.05555552.9633.5003.9504.2804.5204.680 9巴比妥钠-盐酸缓冲液（18）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M巴比妥钠溶液(毫升0.2N盐酸(毫升6.87.07.27.47.67.88.08.2100

12、10010010010010010010018.417.816.715.313.411.479.397.218.48.68.89.09.29.49.61001001001001001001001005.213.822.521.651.130.700.35巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升 10Tris盐酸缓冲液（0.05M，25）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。pHX(毫升pHX(毫升7.107.207.307.407.507.607.707.807.908.0045.744.743.442

13、.040.338.536.634.532.029.28.108.208.308.408.508.608.708.808.9026.222.919.917.214.712.410.38.57.0三羟甲基氨基甲烷（Tris）HOCH2 CH2OHCHOCH2 NH2分子量=121.14;0. 1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。 11硼酸硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.47.67.88.01.01.52.03.09.08.58.07.08.28.4

14、8.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O7·H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。 11硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.47.67.88.01.01.52.03.09.08.58.07.08.28.48.79.03.54.56.08.06.55.54.02.0硼砂Na2B4O7·1

15、0H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3，分子量=61.84, 0.2M溶液为12.37克/升。硼砂 易失去结晶水，必须在带塞的瓶中保存。 12甘氨酸氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升pHXYpHXY8.68.89.09.29.450505050504.06.08.812.016.89.69.810.010.410.6505050505022.427.232.038.645.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。 13硼砂-氢氧化钠缓冲

16、液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升pHXYpHXY9.39.49.65050506.011.023.09.810.010.150505034.043.046.0硼砂Na2B4O7·10H2O,分子量=381.43;0.05M溶液为19.07克/升。 14碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg2+存在时不得使用pH0.1MNa2CO3(毫升0.1MN2HCO3(毫升20379.169.409.519.789.9010.1410.2810.5310.838.779.129.409.509.729.9010.0810.

17、2810.57123456789987654321Na2CO2·10H2O分子量=286.2;0.1M溶液为28.62克/升。N2HCO3分子量=84.0;0.1M溶液为8.40克/升。 15“PBS”缓冲液PH7.67.47.27.0H2ONaClNa2HPO4NaH2PO410008.52.20.110008.52.20.210008.52.20.3100ml8.5g2.2g0.4gPH计校正溶液配置的标准方法1）pH4，邻苯二甲酸氢钾标准缓冲液：精密称取在115±5干燥23小时的邻苯二甲酸氢钾KHC8H4O410.12g，加水使溶解并稀释至1000ml。 2）pH7，磷酸盐标准缓冲液(pH7.4：精密称取在115±5干燥2