讲义文稿案例connective tissue

1、Connective Tissue 1.To understand the structure of loose connective tissue.2.To understand the difference between loose connective tissue and dense connective tissue. SlidesLoose connective tissue (No.4, No.1 )Dense connective tissue (No.14)Adipose tissue (No.14/ No.5)Reticular tissue (No.6)1. Gener

2、al features: 1)small number of cells and large amount of extracellular matrix (intercellular material). 2)the extracellular matrix is composed of fibers and an amorphous ground substance. 3) functions: connection, supporting, protecting, nutrition, defense and repairing.The types of CT are determine

3、d on the basis of the types of cell and the characteristics of the extracellular matrix.CT in narrow sense means connective tissue proper, include: loose CTadipose tissuereticular tissuedense CTLCT：HE X400Material: rabbit mesenterySlide No:4High power:1. Elongated and tortuous collagenous fibers are

4、 stained red and arranged in bundles.2. The elastic fibers are blue stained, branched and formed a woven network.3. The fibroblast is the cell most commonly in close to the collagen fiber. Its basophilic nucleus is visible.4. For the macrophage, its cytoplasm contains some blue dye substance.Loose c

5、onnective tissueFibroblastMacrophageSmall intestine: HE X40Slide No:1Method: H.Elower power:The lightly stained submucosa between mucosa and smooth muscle layer.Small intestine: LCT HE X400High power:1.Collagenous fiber stained in red distributed in bundles, thick or thin.2.The fibroblast is flat. I

6、ts cytoplasm is stained lightly so the boundary is not clear.3.The macrophages are not easily to be distinguished.Refractable elastic fiberSkin :DCT HE X40Dense connective tissue and adipose tissueSlide No:14Material: human skin from fingerMethod: H.ENaked eye: in the section, epidermis is violent s

7、tained. Under epidermis, the dermis is pink and composed of dense connective tissue.In hypodermis, you can find loose connective tissue and adipose tissue.High power:1.Collagenous fibers are dense, arranged irregularly and formed thick bundles.2.The numbers of the cells are few, most of which are fi

8、broblasts and fibrocytes.Hypodermis is loose tissue. 3.A lot of fat cell pile up and form adipose tissue.Adipose tissueAdipose tissue HE X400The fat cell is vacuolated. The cell nucleus was pushed to side. Lymph node: Reticular tissue HE X400Reticular tissueSlide No.5Material: lymph node of catMetho

9、d: H.EHigh power:Reticular cell: A reticular cell produces reticular fiber and surrounds the fibers with its cytoplasm.It has many processes. Its nuclei is lightly stained, but the nucleolus is clear.Lymph node: Reticular tissue HE X400Lymph node: Reticular tissue Silver X100Lymph node: Reticular ti

10、ssue Silver X400FibroblastMacrophage Mast cellPlasma cellCollagenous fiberscollagenous fibrilfibroblastPlasma cellMast cellmacrophagyThe drawingLoose connective tissue H&E stain 400MacrophageCollagenous fiberElastic fibersFibroblastHomework:Drawing：Loose connective tissue ( No.4 ) 巨噬细胞 (macrophage)

11、成纤维细胞 (fibroblast) 胶原纤维 (collagenous fiber) 弹性纤维 (elastic fiber)Trachea：Hyaline Cartilage HE X40Hyaline cartilageSlide No:3Material: human tracheaMethod: H.E1. Hyaline cartilage is covered by a layer of dense connective tissue called perichondrium.2. It is rich in collagen fibers and contain cells:

12、fibroblast; undifferentiated mesenchymal cells, chondroblasts and chondrocytes.refraction coefficientTrachea：Hyaline Cartilage HE X4003. Isogenous groups4. Cartilage lacuna is the chondrocytes location5. Cartilage capsule: around every chondrocyte, it contain some basophilic substance.(chondroitin s

13、ulfuric acid )The auricle of the ear: Elastic cartilage Weigert X400Fibrocartilage Hematoxylin X100 (tendon )Fibrocartilage Hematoxylin X400Bone：HE X100BoneSlide No:7Material: human long boneMethod: grinding slideLow power:1.Osteocyte2.Bone lacuna and bone canaliculus3.Osteon4. Circumferential lamella, interstitial lamellaSilver nitrateArranged on the concentric circles Bone：HE X