Sulforhodamine-101-SR101-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESulforhodamine 101Cat. No.: HY-101878CAS No.: 60311-02-6Synonyms: SR101分式: CHNOS分量: 606.71作靶点: Others作通路: Others储存式: -20C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性数据体外实验 DMSO : 75 mg/

2、mL (123.62 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.6482 mL 8.2412 mL 16.4823 mL5 mM 0.3296 mL 1.6482 mL 3.2965 mL10 mM 0.1648 mL 0.8241 mL 1.6482 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保

3、证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.12 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.12 mM); Clear solution1/2 Master of Small Molec

4、ules 您边的抑制剂师www.MedChemEBIOLOGICAL ACTIVITY物活性 Sulforhodamine 101种红荧光染料。体外研究 Sulforhodamine 101 does not label astrocytes in brainstem slices as strong and specific as in thehippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has tobe kept as low as po

5、ssible or the labeling procedure can be performed after the actual experiment 1.体内研究 In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 MSulforhodamine 101 or topical application of 100 M 1.PROTOCOLCell Assay 1 Acute brain slices are usually incubated in

6、 carbonated extracellular solution containing 0.5 to1 MSulforhodamine 101 for 20 to 30 min and 34 to 37C. Following this, excess dye is removed over a period of10 to 30 min using different protocols that were described earlier 1.MCE has not independently confirmed the accuracy of these methods. They

7、 are for reference only.Animal For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 MAdministration 1 or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has beenreported to be successful 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Hlsmann S, et al. Limitations of Sulforhodamine 101 for Brain Imaging. Front Cell Neurosci. 2017 Feb 28;11:44.McePdfHeightCaution: Product has not been fully validated for medical applications.For