Polydatin-Piceid-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPolydatinCat. No.: HY-N0120ACAS No.: 27208-80-6Synonyms: Piceid分式: CHO分量: 390.38作靶点: Autophagy; Apoptosis; Mitophagy作通路: Autophagy; Apoptosis储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO :

2、29 mg/mL (74.29 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.5616 mL 12.8080 mL 25.6161 mL5 mM 0.5123 mL 2.5616 mL 5.1232 mL10 mM 0.2562 mL 1.2808 mL 2.5616 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Polydatin提取从传统中药虎杖根，在多个

3、实验模型中具有抗炎作。IC50 & Target NF-B, Autophagy体外研究Polydatin protects cerebral cells from ischemic damages via improvement of microcirculation and inhibition of1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEplatelet aggregation. In addition, polydatin inhibits ICAM-1 expression in endothelial cells stimu

4、lated bylipopolysaccharide; it also attenuates adhesion between white blood cells and endothelial cells 1.体内研究 Polydatin could significantly increase the activity of SOD and the heart rate, attenuate myocardialpathological damage, decrease MDA content, slightly increase arterial pressure and GSH-Px

5、activity, reduceintervals of QRS, QT and ST, and lower FFA content 2.PROTOCOLCell Assay 3 The effect of polydatin on mMEC viability is evaluated with an MTT assay. mMECs are incubated in thepresence or absence of various concentrations of polydatin (25, 50 and 100 g/mL) and DEX (100 g/mL) for24 h. N

6、ext, 20 L of MTT (5 mg/mL) is added to each well and incubated for 4 h. After the supernatants areremoved and the formazan is dissolved with 150 L of DMSO in each well, the optical density (OD) value ismeasured at 570 nm on a microplate reader 3.MCE has not independently confirmed the accuracy of th

7、ese methods. They are for reference only.Animal Rats: Rats are divided into six groups by random assignment and treated as follows: in normal group andAdministration 23 polydatin control group: rats are administrated with CMC-Na and polydatin (200 mol/kg) by gavagerespectively, and given normal sali

8、ne (NS) by tail intravenous (iv) injection with the same volume; in DOXgroup: rats are injected with DOX by cauda vein for 4 weeks (3 mg/kg per week), the cumulative dosage is12 mg/kg similar to that in the research of Chang et al 2.Mice: Polydatin is dissolved in dimethyl sulfoxide (DMSO) and dilut

9、ed in Dulbeccos modified Eagles medium(DMEM). Sixty adult female postpartum and lactating BALB/c mice (68 weeks old, weighing 3540 g) areobtained. Control group (CG): The mice are treated with 100 L of PBS as a vehicle control. Polydatingroups: 24h after S aureus infection, the mouse model of S aure

10、us mastitis is intraperitoneally administeredpolydatin at 15, 30 and 45 mg/kg 3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Cheng Y, et al. Involvement of cell adhesion molecules in polydatin protection of brain tissues from ischemia-re

11、perfusioninjury. BrainRes. 2006 Sep 19;1110(1):193-200.2. Wang HL, et al. Synergistic effects of Polydatin and Vitamin C in Inhibiting Cardiotoxicity induced by Doxorubicin in rats. Fundam ClinPharmacol. 2016 Nov 28. Epub ahead of print3. Jiang KF, et al. Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38MAPK/NF-B pathway. Acta Pharmacol Sin. 2016 Nov 28. Epub ahead of printMcePdfHeightCaution: Product h