单细胞DNA甲基化分析课件

1、单细胞DNA甲基化分析occurs at the carbon-5 position of cytosine residue of the CpG dinucleotideRelated to X-chromosome inactivation, imprinting, and maintenance of chromosomal stabilityschizophrenia, bipolar disorder, Alzheimers disease, diabetes, atherosclerosis, cancer, major psychosis, lupus and Parkinson

2、s disease分离单细胞单细胞DNA甲基化甲基化分析分离单细胞流式细胞分析酶解法微流体设备激光捕获显微切割技术用毛细管等器具手动获取甲基化分析重亚硫酸盐胞嘧啶尿嘧啶PCR富集测序分析那为何单细胞DNA甲基化分析直到近些年才成为研究热点了呢？enzyme-based single-cell methylation assay (RSMA)参考文献Martin Kantlehner,Roland Kirchner.A high-throughput DNA methylation analysis of a single cell. Nucleic Acids Research, 2011,

3、Vol. 39, No. 7 e44;C. Lorthongpanich, L.F. Cheow, S. Balu, S.R. Quake, B.B. Knowles, W.F. Burkholder, D. Solter, D.M. Messerschmidt.Single-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos.Science, 341 (2013), pp. 11101112;Eun-Joon Lee, Junfeng Luo. Analyzing the

4、cancer methylome through targeted bisulfit sequencing. Cancer Lett. 2013 November 1; 340(2);Sbastien A Smallwood, Heather J Lee. single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity. nature methods, 2014, VOL.11,NO.8, pp. 817820;Matthias Farlik,Nathan C. SheffieldSingle-Cell DNA Methylome Sequencing and Bioinformatic Inference of Epigen