参松养心胶囊抗心律失常机理研究ppt1

参松养心胶囊抗心律失常机理研究

Studyonthemechanismofantiarrhythmiceffects

ofShensongYangXin

药学班程义如ABSTRACT

Cardiacarrhythmiaisakindofcommoncardiovasculardiseasesintheclinicalpractice，whichhavegreateffectsonlifequalityandsafetyofthePatients.AtPresent,antiarrhythrmicdrugshavebeenclassifiedassodiumchannelblockers，β-recepterbloekers，actionPotentialdurationProlongagentsandcalciumchannelblockers.Althoughtheclassificationofantiarrhythmicdrugsaredifferent,modulationtheionchannelsfunctionisthebasicmechanism.摘要心律失常是临床常见且对患者的生活质量和生命安全影响极大地心血管疾病。目前，抗心律失常西药主要包括钠离子通道阻滞剂，β受体阻断剂、延长动作电位时程药和钙离子通道阻断剂。尽管药物分类不同，其作用机制有差异，归根到底是通过对各种心肌细胞离子通道功能的调节来起作用的。Comparedtowesternmedicine,someantiarrhythmicChineseherbswithmanycomponentscanmulti一targetmodulatethedisorderofionchannelfunction.Itmightexertantiarrhythmiceffortswithlowerincidenceofadverseeffects.同西药相比，抗心律失常作用的中药不仅对心脏离子通道功能有影响，而且中药往往是复合药物，具有多离子通道靶点的作用，因而毒性低副作用小。ShensongYongxin(SSYX)isoneofthecompoundrecipeofChinesemateriamedicalincluding12ingredients.PreviousstudiesonanimalmodelshowedthatSSYXsignificantlyinhibitedthearrhythmiasicinducedbytoxicchemicalcompoundsorischemia一reperfusioninjury.Smallrandomdouble一blindclinicaltrialsalsosuggestedthatSSYXreducedthenumberofventricularextrabeatsinpatientswithorwithoutstructureheartdisease参松养心胶囊(SSYX)是一种复方制剂，方中多数药物均有较好的抗心律失常作用。动物实验证明，SSYX可以明显抑制有毒化学药物或者局部灌注损伤缺血诱发的心律失常;随机临床验显示，SSYX对治疗冠心病心律失常有明显疗效。ObjiectivesTodeterminetheeffectsofShensongYangXin(SSYX)onL一tyPecalciumchannels(ICa，L)，sodiumchannels(INa)，transientoutwardpotassiumcurrent(It)，delayedrectifiercurrent.目的观察ssyx干粉提取溶液对单个心室肌细胞L型钙电流(Ica，:)，钠电流(东a)，瞬时外向钾电流(It。)，内向整流钾电流(众1)，延迟整流钾电流慢成分(反s)的影响，resultsTheactionpotentialandioncurrentcouldberecordedintheisolatedventricularmyocytesofguineapigsandrats，whichindicatedthemyocyteshadsatisfaetoryelectrophysiologicalproperties.Approximate40一60percenttransfectedHEK293cellstookgreenfluorescence，inwhichIkrcould