分子生物学Molecular Biology智慧树知到期末考试答案章节答案2024年山东第一医科大学_第1页
分子生物学Molecular Biology智慧树知到期末考试答案章节答案2024年山东第一医科大学_第2页
分子生物学Molecular Biology智慧树知到期末考试答案章节答案2024年山东第一医科大学_第3页
分子生物学Molecular Biology智慧树知到期末考试答案章节答案2024年山东第一医科大学_第4页
分子生物学Molecular Biology智慧树知到期末考试答案章节答案2024年山东第一医科大学_第5页
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分子生物学(MolecularBiology)智慧树知到期末考试答案+章节答案2024年山东第一医科大学mRNAisthemostabundanttypeinlivingcells(bypercent)?()

答案:对RNAPolIIistheenzymesynthesizingRNAinmammals.Itisnotsusceptibletoamanitin.()

答案:错DNAsequencesandDNA-bindingproteinscontroltranscription.TheDNAsequencesarenamedascis-actingelements,suchastheTATAbox,enhancersandotherelements.()

答案:对MismatchrepairsystemcanincreasetheDNAreplicationfidelityby100-1000times.()

答案:对BothgenomicDNAandcDNAcanbeusedastargetDNAinmolecularcloning.()

答案:对BothprokaryoticandeukaryoticDNApolymerasesarecomposedofonepolypeptidechain.()

答案:错GenechipcanalsobecalledDNAmicroarray.()

答案:对Intheeukaryotictrans-actingfactors,activatorproteinsusuallybindtothepromotersdirectly.()

答案:错Stopcodonsarenevertranslated.()

答案:错Theproteinproductcodedbymycgeneistranscriptionfactor.()

答案:对Plasmidscanaccommodateupto45kbforeignDNA.()

答案:错RibosomesbecomeattachedtotransferRNA(tRNA)moleculesbeforethetranslationprocesscanbegininProkaryotes.()

答案:错OnlytheenvironmentalagentscaninducetheoccurrenceofDNAdamages.()

答案:错Trans-actingtranscriptionfactorsaretheDNA-bindingproteinsthatbindwithhighspecificitytoDNAregulatorysequencesandfacilitatethebindingandpositioningofRNAPolforthesynthesisofpre-RNA.()

答案:对Bothprokaryoticandeukaryotictranscriptionsareaccompaniedbytranslation.()

答案:错ThebaseinDNA_____

答案:A;C;G;TtRNAinprokaryoticcellcontains()

答案:16sRNA;23sRNA;5sRNAtRNAineukaryoticcellcontains_____

答案:5.8sRNA;18sRNA;28sRNA;5sRNAEF-TuandEF-Tsareinvolvedinproteinbiosynthesis:()

答案:Peptidechainextension;Ser-tRNAserentryWhatarethelipoproteinsobtainedinagarosegelelectrophoresisofbloodlipoproteinexperiment?()

答案:αlipoprotein;βlipoproteinReversetranscriptasecan()

答案:synthesizeDNAusingDNAastemplate;synthesizeDNAusingRNAastemplateTheadvantagesofprokaryoticexpressionsysteminclude()

答案:Highexpressionleveloftargetgene;Suitableforlarge-scaleproduction;ShortcultureperiodWhichofthefollowingstepscouldberegulatedtoinfluencetheamountoftheproductofageneineukaryotes?()

答案:Activationofchromosomes;mRNAdegradation;Post-transcriptionalmodification;TranscriptioninitiationProteinsynthesisinprokaryoticcellsischaracterizedby:()

答案:Transcribeandtranslateatthesametime;Therearethreeterminationfactors;ThestartingtRNAistRNAmetWhichofthefollowingaretrueabouttheenhancers?()

答案:Enhancerscanliefarawayfromgene;EnhancerscanbelocatedupstreamordownstreamofthegeneThefinalproductsofgeneexpressioninclude:()

答案:rRNA;protein;tRNAInDNA,phosphodiesterbondsformwithhydroxylgroupsofcarbonsnumber________and________ofeachdeoxyribosesugar.()

答案:3ʹ;5ʹWhichoftheclassesofRNAmoleculesis/arepartoftheprocessoftranslation?()

答案:TransferRNA;RibosomalRNA;MessengerRNAThebaseinDNA()

答案:G;A;C;TTheRhoproteinisinvolvedinthe_____stageoftranscription.()

答案:terminationA“mini-gene”hasthebasesequenceTACCCGTGCACG.IftheTatthebeginningofthesequenceisdeleted,whatwillbetheconsequence?()

答案:Allofthecodonsafterthatpointwillbechanged.WhatarethestepsofPCR?()

答案:Denaturation;Extension;AnnealingWhichofthefollowingoccursfirstinprokayotictranscription?()

答案:formationofaclosedpromotercomplexOfallthepossibleaminoacids,alllivingorganismsmakeuseofonlythesame20aminoacids.Thissupportstheideathat:()

答案:AlllivingthingsarederivedfromacommonancestorAmongthestatementsabout3’poly(A)tailwhicharecorrect?()

答案:ItcanprotectmRNAfromdegradation.;Someeukaryoticproteinshaveno3’poly(A)tailontheirmRNAs.;ItcanenhancetranslatabilityofmRNA.DNAmoleculesUsedasprobesmust:()

答案:denaturebeforehybridizationIninsertionalinactivationselection,Whichofthefollowingbacteriacangrowinthepresenceoftetracycline()

答案:BacteriatransformedwithwildvectorThetoolenzymeusedforcombinationofforeignDNAandvectoris()

答案:DNAligaseWhatisthemoleculesizeofthetargetbandofPCR?()

答案:640bpAmountofTinRNAis0.25mol,soamountofAis_____.

答案:0.25molThesecondarystructureofDNA

is

_____

答案:doublehelixWhichcanrecognizetheN-terminusofthenascentpeptidechainofthesecretedprotein?()

答案:SignalrecognitionparticleWhichofthefollowingisthemostessentialforageneexpression?()

答案:promoterWhatistheextensiontemperatureofthePCR?()

答案:72℃tRNAisusedas_____intranslation.()

答案:adaptorTheShine-Dalgarnosequenceisfoundonthe().

答案:mRNAWhichenzymebelowcannotform3’,5’-phosphodiesterbond?()

答案:helicaseWhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair?()

答案:ThereisnoanyDNAsynthesisInα-complementationselection,theforeignDNAwillbeinsertedinto()

答案:LacZ’geneWhichbasesubstitutionbelowbelongsto“Transition”pointmutation?()

答案:C→TwhichbondanaminoacidislinkedtothetRNA?()

答案:esterThetechnologyofisotope-labeledprobestodetecttheRNAmoleculeontheNCmembraneiscalled:()

答案:NorthernBlottingmRNA

isusedas_____intranslation.

答案:templatePleasearrangethefollowingproteinsbytheorderofparticipatinginDNAreplication()①Primase②Helicase③Single-strandbindingproteins④DNApolymeraseI

答案:②③①④Whichbandrunsfastestintheagarosegelelectrophoresisofbloodlipoproteinexperiment?()

答案:αlipoproteinWhichregulatorcouldinhibitsgenetranscription?()

答案:silencerWhichofthefollowingisexpressedatrelativelyconstantrateinalmostallthecells?()

答案:housekeepinggeneWhichisthemostcommonlyusedelementsofeukaryoticpromoter?()

答案:TATAboxWhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()

答案:TFIIDWhichoneofthefollowingistumorsuppressorgene?()

答案:RbAccordingtoChargaffʹsobservationsofnucleotidecompositionofDNAsamples()

答案:AlloftheaboveWhichisFALSEdescriptionofDNAreplication()

答案:EukaryoticDNAcontainonlyonerepliconWhichisnottrueofthedifferentconformationsofDNA?()

答案:BothA-DNAandB-DNAaredehydrated.WhatwouldbethetRNAanticodonforthemRNAcodonCAG?()

答案:CUGInthepeptidechainsynthesis,whichsiteaminoacyl-tRNAboundwiththeprokaryoticribosome?()

答案:AsiteWhichofthefollowingoccursasaresultoftranslocation?()

答案:thetRNAthatwasinthePsitemovesintotheEsiteIntheregulationoftrpoperon,whenthetryptophanisscarceinmedium,whichstatementistrue?()

答案:Repressorproteinisinactive,attenuatorwouldn’tformWhichproteinfactorhastranslocaseactivityinprokaryotes?()

答案:EFGWhichstatementistrue?()

答案:DuringthesplicingintronscanformthelariatRNA.InwhichprocessGG-NERandTC-NERshowdifferent()

答案:DNAlesionrecognitionStartingwith15N15N(heavy)DNA,andafter3generationsinthe14Nmedium,E.colicellswillproducethefollowingband(s)indensity-gradientcentrifugationasbelow:()

答案:onebandoflightDNAandonebandofintermediateDNAInlacoperon,whatistherightorderofthethreeregulatorysequences?()

答案:CAPsite-promoter-operatorThecorrespondingcodontoanticodonUCCis()

答案:GGAAmongthefollowingfactors,theonethatdoesnotparticipateinthetranslationprocessofprokaryotesis()

答案:eEF1Duringproteinsynthesis,theactivationsiteofaminoacidsis()

答案:αcarboxylWhichofthefollowingsequencesispalindromesequence()

答案:AGTATACTAmountofGinRNAis0.25mol,soamountofCis().

答案:0.25mol【答案】Whichofthefollowingisanadvantageofphagevectorsoverplasmidvectors?()

答案:TransfectionismoreefficientthantransformationforlargerpiecesofDNAWhichenzymeelongatestheleadingstrandinE.coli?()

答案:DNApolymeraseIIIAlltheRNAsbelowaretranscribedbyRNApolIexcept()

答案:5SrRNAMitochondrialDNAisreplicatedby()

答案:D-loopreplicationWhichRNApolymerasetranscribesthegeneof28SrRNA?()

答案:RNApolymeraseIInmostmelanoma,oncogenicBRAFwasactivatedby()

答案:MutationWhatthesignalpeptideacts?()

答案:GuidethepolypeptidechainthroughtheroughendoplasmicreticulumchannelintotheendoplasmicreticulumlumenWhichofthefollowingeukaryoticligasesinvolvesinfillingthegapbetweentheOkazakifragments()

答案:LigaseIWhatistheorderoflipoproteinfromthecathodetoanode?()

答案:CM,βlipoprotein,pre-βlipoprotein,αlipoproteinWhatarethesolutionsusedintheexperiment?()

答案:Isopropanol;TEbuffer;Phenol/chloroform/Isopropanol(25/24/1);70%ethanolWhatisthebacterialusedintheexperiment?()

答案:DH5αWhatarethestepsofPCR?()

答案:Denaturation;Extension;AnnealingWhatistheannealingtemperatureofthePCR?()

答案:55℃Whatisthestainingreagentintheexperiment?()

答案:SultanblackdyeInblue/whitescreening,thebacteriacontainingwildvectorswillyieldwhitecolonies.()

答案:错RestrictionenzymesrandomlycleaveDNA()

答案:错Plasmidsareusuallyintroducedintobacterialcellsby.()

答案:TransformationBasedonthenamingrules,thefirstletterofarestrictionendonucleasemeans()

答案:BacterialgenusIsocaudarnerpresents()

答案:Variousrestrictionendonucleasesrecognizedifferentpalindromes,butproducesamestickyendsWhichisnotnecessaryaspartofprokaryoticexpressionvector?()

答案:PolyAsignalIninsertionalinactivationscreening,thebacteriathataresensitivetotetracyclineandresistanttoampicillinshouldbetransformedwith()

答案:RecombinantDNAT4DNAligaserequires()

答案:ATPWhichofthefollowingDNAmoleculesarecommonlyusedinmolecularcloning()

答案:BAC;Plasmid;Bacteriophage;CosmidWhichofthefollowingmethodsisusedforisolationofforeignDNA()

答案:Chemicalsynthesis;Yeasttwo-hybridsystem;DNAlibrary;PCRTheloadingcapacityofplasmidsisusually()

答案:Lessthan10kbWhichofthefollowingstatementsisnottrueconcerningTypeIIrestrictionendonucleases?()

答案:TheycancleavesingleDNAWhichtechnologycanbeusedtoidentifyDNA?()

答案:SouthernblottingInthePCRreaction,thepurposeofannealingistomakethecombinationofprimerandtemplate.()

答案:对ThebasiccomponentsofthePCRsystemdoesnotinclude:()

答案:NTPWhicharerightaboutmolecularhybridization?()

答案:Thehybridmoleculesmustbesingle;FromtheunderstandingofDNAdenaturationandrenaturationphenomenonProteinchiptechnologyhasthecharacteristicsoffastandhighthroughput,itcanbeusedtoanalyzethousandsofproteinsinthewholegenomelevelatthesametime.()

答案:对ThebasicstepsofPCRreactionare:()

答案:denaturation,annealing,extensionThemechanismsoftumorsuppressorgeneinactivationinclude()

答案:hypermethylation;Mutation;LossofheterozygosityInmostchronicmyelogenousleukemia,tumorigenesisiscausedbyoncogeneamplification()

答案:错Thefollowingchangespromoteoncogeneactivation,except()

答案:LossofheterozygosityWhichstatementistrueaboutoncogenes?()

答案:TheyareusuallyactivatedincancerdevelopmentWhichoneofthefollowingisoncogene?()

答案:mycTheactivechromatinusuallyshowslowlevelofmethylation.()

答案:对Enhancersmustlocateontheupstreamregionofpromoter.()

答案:错Ifageneisexpressedonlyincertainorgansortissues,thisiscalled()

答案:spatialspecificityofgeneexpressionIntheregulationoflacoperon,whenthemediumcontainsbothglucoseandlactose,whichstatementistrue?()

答案:bothrepressorandCAPareinactiveIfageneisexpressedatrelativelyconstantrateinalmostallthecells,itiscalleda(an)()

答案:housekeepinggeneWhichoneisNOTacis-actingelement?()

答案:activatorproteinInlacoperon,repressorproteincouldbindto()sequence.()

答案:operatorThemostefficientstepofgeneexpressionregulationis()

答案:transcriptioninitiationWhichisNOTafinalproductofgeneexpression?()

答案:mRNAIntheregulationoflacoperon,whenthemediumcontainsonlyglucose,whichstatementistrue?()

答案:concentrationofcAMPislow,CAPisinactiveTheprokaryoticoperatorsequenceisusually().

答案:locatedontheupstreamregionofpromoterWhichisNOTthebasalcis-actingelementsineukaryotes?()

答案:operatorThegeneticcodehasfourletters,A,T,G,andC.()

答案:错Polypeptidesunfoldinthecytoplasmofacelltoformafunctionalprotein.()

答案:错MessengerRNA(mRNA)ismadebyaprocesscalledtranslation.()

答案:错ThespecificsequenceontheNterminusofsecretoryproteiniscalled()

答案:signalpeptideWeusuallycallcodonscontains5characteristics,amongwhichthedegeneracyofthegeneticcodemeans?()

答案:MostaminoacidshavemorethanonesetofcodonsWhichproteinfactorhasthetranslocaseenzymeactivityineukaryotes:()

答案:eEF-2proteinfactorAmongthefollowingfactors,whichonecoulddelivertheaminoacyl-tRNAtotheAsiteofribosome?()

答案:EF-TuTheShine-Dalgarnosequenceisfoundonthe________.()

答案:mRNATheenergyrequiredforthestepofpeptidechainelongationinprokaryoticis?()

答案:onlyGTPAgenehasthebasesequenceACCCGTGCACGT.IftheAatthebeginningofthesequenceisdeleted,whatwillbehappen?()

答案:AllofthecodonsafterApointwillbechangedWhichbondisanaminoacidlinkedtothetRNA?()

答案:esterEukaryotictranscriptionsisaccompaniedbytranslation.()

答案:错RNAPolIIistheenzymesynthesizinghnRNAineukaryotes.Itisnotsusceptibletoamanitin.()

答案:错WhichRNApolymerasetranscribesthegeneofinsulin?()

答案:RNApolymeraseIIThefunctionsof5’CapofeukaryoticmRNAinclude()

答案:enhancementofthemRNA’stranslatability;;protectionofthemRNAfromdegradation;;transportofthemRNAfromnucleustocytosol;Cis-actingelementsandtrans-actingfactorscontroltranscription.Thecis-actingelementsincludetheTATAbox,enhancersandotherelementsetal.()

答案:对Inprokaryotes,RNApolymerasecanbindupstreamsequencesofpromotersdirectly.Eukaryotesneedsomeproteinstofacilitatethebinding.Whatarethenamesoftheseproteins?()

答案:TranscriptionfactorsAmutationtothissequenceineukaryoticmRNAwillaffecttheprocessbywhichthe3'-endpoly-AtailisaddedtothemRNA.()

答案:AAUAAAWhichofthefollowingstepsoccursfirstinprokayotictranscription?()

答案:formationofaclosedpromotercomplexWhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()

答案:TFIIDWhichcomponentoftheprokaryoteRNApolymeraseholoenzymeisinvolvedinrecognizingpromotersspecifictocertaintypesofgenes?()

答案:σWhichtypeofDNApointmutationswillnotleadtothechangeinaminoacid().

答案:SilentmutationDuringmismatchrepairinprokaryotes,thenewlysynthesizedDNAisrecognizedbythelackof()modification()

答案:Methylationwhichbasesubstitutionbelowbelongsto“Transition”pointmutation.()

答案:C→TDNAdamageisthemaincausefortheincreasedfrequencyofgenemutation.()

答案:对InwhichprocessGG-NERandTC-NERshowdifferent()

答案:DNAlesionrecognitionBaseexcisionrepairisoneofDNArepairpathwaystocorrectslargebaselesions.()

答案:错ThedeficiencyofNERrepairproteinsissusceptibleto_disease.()

答案:XerodermapigmentosumWhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair.()

答案:ThereisnoanyDNAsynthesisWhichofthefollowingDNAdamagesbelongstoexogenousDNAdamage().

答案:IonizingradiationDuringdoublestrandedDNAbreakrepair,NHEJshowsmoreprecisecomparedtoHR.()

答案:错TheOkazakifragmentofeukaryotesislongerthanthatofprokaryotes.()

答案:错ReversetranscriptaseexertsthefunctionsofRNAdependentDNApolymeraseandDNAdependentDNApolymerase.()

答案:对Whichmechanismcanexplaintwostrandsaresynthesizedintheoppositedirection,inthereplicationfork?()

答案:semidiscontinuousreplicationRNAprimerinDNAreplication()

答案:provides3’-OHtosynthesizeDNA;isrequiredbyallOkazakifragmentsWhatarethedifferencesofeukaryoticDNAreplicationfromprokaryotes?()

答案:thereplicationoriginofeukaryotesisshorterthanthatinprokaryotes;eukaryoticDNAcontainsmultipleorigins;eukaryoticDNAisreplicatedinSphaseofcellcycle;telomereandtelomeraseaidterminationofeukaryotesWhichis

FALSEabouttheterminationofprokaryoticDNAreplication?()

答案:CoordinatethesynthesisoflaggingandleadingstrandsAlthough

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