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分子生物学(MolecularBiology)智慧树知到期末考试答案+章节答案2024年山东第一医科大学mRNAisthemostabundanttypeinlivingcells(bypercent)?()
答案:对RNAPolIIistheenzymesynthesizingRNAinmammals.Itisnotsusceptibletoamanitin.()
答案:错DNAsequencesandDNA-bindingproteinscontroltranscription.TheDNAsequencesarenamedascis-actingelements,suchastheTATAbox,enhancersandotherelements.()
答案:对MismatchrepairsystemcanincreasetheDNAreplicationfidelityby100-1000times.()
答案:对BothgenomicDNAandcDNAcanbeusedastargetDNAinmolecularcloning.()
答案:对BothprokaryoticandeukaryoticDNApolymerasesarecomposedofonepolypeptidechain.()
答案:错GenechipcanalsobecalledDNAmicroarray.()
答案:对Intheeukaryotictrans-actingfactors,activatorproteinsusuallybindtothepromotersdirectly.()
答案:错Stopcodonsarenevertranslated.()
答案:错Theproteinproductcodedbymycgeneistranscriptionfactor.()
答案:对Plasmidscanaccommodateupto45kbforeignDNA.()
答案:错RibosomesbecomeattachedtotransferRNA(tRNA)moleculesbeforethetranslationprocesscanbegininProkaryotes.()
答案:错OnlytheenvironmentalagentscaninducetheoccurrenceofDNAdamages.()
答案:错Trans-actingtranscriptionfactorsaretheDNA-bindingproteinsthatbindwithhighspecificitytoDNAregulatorysequencesandfacilitatethebindingandpositioningofRNAPolforthesynthesisofpre-RNA.()
答案:对Bothprokaryoticandeukaryotictranscriptionsareaccompaniedbytranslation.()
答案:错ThebaseinDNA_____
答案:A;C;G;TtRNAinprokaryoticcellcontains()
答案:16sRNA;23sRNA;5sRNAtRNAineukaryoticcellcontains_____
答案:5.8sRNA;18sRNA;28sRNA;5sRNAEF-TuandEF-Tsareinvolvedinproteinbiosynthesis:()
答案:Peptidechainextension;Ser-tRNAserentryWhatarethelipoproteinsobtainedinagarosegelelectrophoresisofbloodlipoproteinexperiment?()
答案:αlipoprotein;βlipoproteinReversetranscriptasecan()
答案:synthesizeDNAusingDNAastemplate;synthesizeDNAusingRNAastemplateTheadvantagesofprokaryoticexpressionsysteminclude()
答案:Highexpressionleveloftargetgene;Suitableforlarge-scaleproduction;ShortcultureperiodWhichofthefollowingstepscouldberegulatedtoinfluencetheamountoftheproductofageneineukaryotes?()
答案:Activationofchromosomes;mRNAdegradation;Post-transcriptionalmodification;TranscriptioninitiationProteinsynthesisinprokaryoticcellsischaracterizedby:()
答案:Transcribeandtranslateatthesametime;Therearethreeterminationfactors;ThestartingtRNAistRNAmetWhichofthefollowingaretrueabouttheenhancers?()
答案:Enhancerscanliefarawayfromgene;EnhancerscanbelocatedupstreamordownstreamofthegeneThefinalproductsofgeneexpressioninclude:()
答案:rRNA;protein;tRNAInDNA,phosphodiesterbondsformwithhydroxylgroupsofcarbonsnumber________and________ofeachdeoxyribosesugar.()
答案:3ʹ;5ʹWhichoftheclassesofRNAmoleculesis/arepartoftheprocessoftranslation?()
答案:TransferRNA;RibosomalRNA;MessengerRNAThebaseinDNA()
答案:G;A;C;TTheRhoproteinisinvolvedinthe_____stageoftranscription.()
答案:terminationA“mini-gene”hasthebasesequenceTACCCGTGCACG.IftheTatthebeginningofthesequenceisdeleted,whatwillbetheconsequence?()
答案:Allofthecodonsafterthatpointwillbechanged.WhatarethestepsofPCR?()
答案:Denaturation;Extension;AnnealingWhichofthefollowingoccursfirstinprokayotictranscription?()
答案:formationofaclosedpromotercomplexOfallthepossibleaminoacids,alllivingorganismsmakeuseofonlythesame20aminoacids.Thissupportstheideathat:()
答案:AlllivingthingsarederivedfromacommonancestorAmongthestatementsabout3’poly(A)tailwhicharecorrect?()
答案:ItcanprotectmRNAfromdegradation.;Someeukaryoticproteinshaveno3’poly(A)tailontheirmRNAs.;ItcanenhancetranslatabilityofmRNA.DNAmoleculesUsedasprobesmust:()
答案:denaturebeforehybridizationIninsertionalinactivationselection,Whichofthefollowingbacteriacangrowinthepresenceoftetracycline()
答案:BacteriatransformedwithwildvectorThetoolenzymeusedforcombinationofforeignDNAandvectoris()
答案:DNAligaseWhatisthemoleculesizeofthetargetbandofPCR?()
答案:640bpAmountofTinRNAis0.25mol,soamountofAis_____.
答案:0.25molThesecondarystructureofDNA
is
_____
答案:doublehelixWhichcanrecognizetheN-terminusofthenascentpeptidechainofthesecretedprotein?()
答案:SignalrecognitionparticleWhichofthefollowingisthemostessentialforageneexpression?()
答案:promoterWhatistheextensiontemperatureofthePCR?()
答案:72℃tRNAisusedas_____intranslation.()
答案:adaptorTheShine-Dalgarnosequenceisfoundonthe().
答案:mRNAWhichenzymebelowcannotform3’,5’-phosphodiesterbond?()
答案:helicaseWhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair?()
答案:ThereisnoanyDNAsynthesisInα-complementationselection,theforeignDNAwillbeinsertedinto()
答案:LacZ’geneWhichbasesubstitutionbelowbelongsto“Transition”pointmutation?()
答案:C→TwhichbondanaminoacidislinkedtothetRNA?()
答案:esterThetechnologyofisotope-labeledprobestodetecttheRNAmoleculeontheNCmembraneiscalled:()
答案:NorthernBlottingmRNA
isusedas_____intranslation.
答案:templatePleasearrangethefollowingproteinsbytheorderofparticipatinginDNAreplication()①Primase②Helicase③Single-strandbindingproteins④DNApolymeraseI
答案:②③①④Whichbandrunsfastestintheagarosegelelectrophoresisofbloodlipoproteinexperiment?()
答案:αlipoproteinWhichregulatorcouldinhibitsgenetranscription?()
答案:silencerWhichofthefollowingisexpressedatrelativelyconstantrateinalmostallthecells?()
答案:housekeepinggeneWhichisthemostcommonlyusedelementsofeukaryoticpromoter?()
答案:TATAboxWhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()
答案:TFIIDWhichoneofthefollowingistumorsuppressorgene?()
答案:RbAccordingtoChargaffʹsobservationsofnucleotidecompositionofDNAsamples()
答案:AlloftheaboveWhichisFALSEdescriptionofDNAreplication()
答案:EukaryoticDNAcontainonlyonerepliconWhichisnottrueofthedifferentconformationsofDNA?()
答案:BothA-DNAandB-DNAaredehydrated.WhatwouldbethetRNAanticodonforthemRNAcodonCAG?()
答案:CUGInthepeptidechainsynthesis,whichsiteaminoacyl-tRNAboundwiththeprokaryoticribosome?()
答案:AsiteWhichofthefollowingoccursasaresultoftranslocation?()
答案:thetRNAthatwasinthePsitemovesintotheEsiteIntheregulationoftrpoperon,whenthetryptophanisscarceinmedium,whichstatementistrue?()
答案:Repressorproteinisinactive,attenuatorwouldn’tformWhichproteinfactorhastranslocaseactivityinprokaryotes?()
答案:EFGWhichstatementistrue?()
答案:DuringthesplicingintronscanformthelariatRNA.InwhichprocessGG-NERandTC-NERshowdifferent()
答案:DNAlesionrecognitionStartingwith15N15N(heavy)DNA,andafter3generationsinthe14Nmedium,E.colicellswillproducethefollowingband(s)indensity-gradientcentrifugationasbelow:()
答案:onebandoflightDNAandonebandofintermediateDNAInlacoperon,whatistherightorderofthethreeregulatorysequences?()
答案:CAPsite-promoter-operatorThecorrespondingcodontoanticodonUCCis()
答案:GGAAmongthefollowingfactors,theonethatdoesnotparticipateinthetranslationprocessofprokaryotesis()
答案:eEF1Duringproteinsynthesis,theactivationsiteofaminoacidsis()
答案:αcarboxylWhichofthefollowingsequencesispalindromesequence()
答案:AGTATACTAmountofGinRNAis0.25mol,soamountofCis().
答案:0.25mol【答案】Whichofthefollowingisanadvantageofphagevectorsoverplasmidvectors?()
答案:TransfectionismoreefficientthantransformationforlargerpiecesofDNAWhichenzymeelongatestheleadingstrandinE.coli?()
答案:DNApolymeraseIIIAlltheRNAsbelowaretranscribedbyRNApolIexcept()
答案:5SrRNAMitochondrialDNAisreplicatedby()
答案:D-loopreplicationWhichRNApolymerasetranscribesthegeneof28SrRNA?()
答案:RNApolymeraseIInmostmelanoma,oncogenicBRAFwasactivatedby()
答案:MutationWhatthesignalpeptideacts?()
答案:GuidethepolypeptidechainthroughtheroughendoplasmicreticulumchannelintotheendoplasmicreticulumlumenWhichofthefollowingeukaryoticligasesinvolvesinfillingthegapbetweentheOkazakifragments()
答案:LigaseIWhatistheorderoflipoproteinfromthecathodetoanode?()
答案:CM,βlipoprotein,pre-βlipoprotein,αlipoproteinWhatarethesolutionsusedintheexperiment?()
答案:Isopropanol;TEbuffer;Phenol/chloroform/Isopropanol(25/24/1);70%ethanolWhatisthebacterialusedintheexperiment?()
答案:DH5αWhatarethestepsofPCR?()
答案:Denaturation;Extension;AnnealingWhatistheannealingtemperatureofthePCR?()
答案:55℃Whatisthestainingreagentintheexperiment?()
答案:SultanblackdyeInblue/whitescreening,thebacteriacontainingwildvectorswillyieldwhitecolonies.()
答案:错RestrictionenzymesrandomlycleaveDNA()
答案:错Plasmidsareusuallyintroducedintobacterialcellsby.()
答案:TransformationBasedonthenamingrules,thefirstletterofarestrictionendonucleasemeans()
答案:BacterialgenusIsocaudarnerpresents()
答案:Variousrestrictionendonucleasesrecognizedifferentpalindromes,butproducesamestickyendsWhichisnotnecessaryaspartofprokaryoticexpressionvector?()
答案:PolyAsignalIninsertionalinactivationscreening,thebacteriathataresensitivetotetracyclineandresistanttoampicillinshouldbetransformedwith()
答案:RecombinantDNAT4DNAligaserequires()
答案:ATPWhichofthefollowingDNAmoleculesarecommonlyusedinmolecularcloning()
答案:BAC;Plasmid;Bacteriophage;CosmidWhichofthefollowingmethodsisusedforisolationofforeignDNA()
答案:Chemicalsynthesis;Yeasttwo-hybridsystem;DNAlibrary;PCRTheloadingcapacityofplasmidsisusually()
答案:Lessthan10kbWhichofthefollowingstatementsisnottrueconcerningTypeIIrestrictionendonucleases?()
答案:TheycancleavesingleDNAWhichtechnologycanbeusedtoidentifyDNA?()
答案:SouthernblottingInthePCRreaction,thepurposeofannealingistomakethecombinationofprimerandtemplate.()
答案:对ThebasiccomponentsofthePCRsystemdoesnotinclude:()
答案:NTPWhicharerightaboutmolecularhybridization?()
答案:Thehybridmoleculesmustbesingle;FromtheunderstandingofDNAdenaturationandrenaturationphenomenonProteinchiptechnologyhasthecharacteristicsoffastandhighthroughput,itcanbeusedtoanalyzethousandsofproteinsinthewholegenomelevelatthesametime.()
答案:对ThebasicstepsofPCRreactionare:()
答案:denaturation,annealing,extensionThemechanismsoftumorsuppressorgeneinactivationinclude()
答案:hypermethylation;Mutation;LossofheterozygosityInmostchronicmyelogenousleukemia,tumorigenesisiscausedbyoncogeneamplification()
答案:错Thefollowingchangespromoteoncogeneactivation,except()
答案:LossofheterozygosityWhichstatementistrueaboutoncogenes?()
答案:TheyareusuallyactivatedincancerdevelopmentWhichoneofthefollowingisoncogene?()
答案:mycTheactivechromatinusuallyshowslowlevelofmethylation.()
答案:对Enhancersmustlocateontheupstreamregionofpromoter.()
答案:错Ifageneisexpressedonlyincertainorgansortissues,thisiscalled()
答案:spatialspecificityofgeneexpressionIntheregulationoflacoperon,whenthemediumcontainsbothglucoseandlactose,whichstatementistrue?()
答案:bothrepressorandCAPareinactiveIfageneisexpressedatrelativelyconstantrateinalmostallthecells,itiscalleda(an)()
答案:housekeepinggeneWhichoneisNOTacis-actingelement?()
答案:activatorproteinInlacoperon,repressorproteincouldbindto()sequence.()
答案:operatorThemostefficientstepofgeneexpressionregulationis()
答案:transcriptioninitiationWhichisNOTafinalproductofgeneexpression?()
答案:mRNAIntheregulationoflacoperon,whenthemediumcontainsonlyglucose,whichstatementistrue?()
答案:concentrationofcAMPislow,CAPisinactiveTheprokaryoticoperatorsequenceisusually().
答案:locatedontheupstreamregionofpromoterWhichisNOTthebasalcis-actingelementsineukaryotes?()
答案:operatorThegeneticcodehasfourletters,A,T,G,andC.()
答案:错Polypeptidesunfoldinthecytoplasmofacelltoformafunctionalprotein.()
答案:错MessengerRNA(mRNA)ismadebyaprocesscalledtranslation.()
答案:错ThespecificsequenceontheNterminusofsecretoryproteiniscalled()
答案:signalpeptideWeusuallycallcodonscontains5characteristics,amongwhichthedegeneracyofthegeneticcodemeans?()
答案:MostaminoacidshavemorethanonesetofcodonsWhichproteinfactorhasthetranslocaseenzymeactivityineukaryotes:()
答案:eEF-2proteinfactorAmongthefollowingfactors,whichonecoulddelivertheaminoacyl-tRNAtotheAsiteofribosome?()
答案:EF-TuTheShine-Dalgarnosequenceisfoundonthe________.()
答案:mRNATheenergyrequiredforthestepofpeptidechainelongationinprokaryoticis?()
答案:onlyGTPAgenehasthebasesequenceACCCGTGCACGT.IftheAatthebeginningofthesequenceisdeleted,whatwillbehappen?()
答案:AllofthecodonsafterApointwillbechangedWhichbondisanaminoacidlinkedtothetRNA?()
答案:esterEukaryotictranscriptionsisaccompaniedbytranslation.()
答案:错RNAPolIIistheenzymesynthesizinghnRNAineukaryotes.Itisnotsusceptibletoamanitin.()
答案:错WhichRNApolymerasetranscribesthegeneofinsulin?()
答案:RNApolymeraseIIThefunctionsof5’CapofeukaryoticmRNAinclude()
答案:enhancementofthemRNA’stranslatability;;protectionofthemRNAfromdegradation;;transportofthemRNAfromnucleustocytosol;Cis-actingelementsandtrans-actingfactorscontroltranscription.Thecis-actingelementsincludetheTATAbox,enhancersandotherelementsetal.()
答案:对Inprokaryotes,RNApolymerasecanbindupstreamsequencesofpromotersdirectly.Eukaryotesneedsomeproteinstofacilitatethebinding.Whatarethenamesoftheseproteins?()
答案:TranscriptionfactorsAmutationtothissequenceineukaryoticmRNAwillaffecttheprocessbywhichthe3'-endpoly-AtailisaddedtothemRNA.()
答案:AAUAAAWhichofthefollowingstepsoccursfirstinprokayotictranscription?()
答案:formationofaclosedpromotercomplexWhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()
答案:TFIIDWhichcomponentoftheprokaryoteRNApolymeraseholoenzymeisinvolvedinrecognizingpromotersspecifictocertaintypesofgenes?()
答案:σWhichtypeofDNApointmutationswillnotleadtothechangeinaminoacid().
答案:SilentmutationDuringmismatchrepairinprokaryotes,thenewlysynthesizedDNAisrecognizedbythelackof()modification()
答案:Methylationwhichbasesubstitutionbelowbelongsto“Transition”pointmutation.()
答案:C→TDNAdamageisthemaincausefortheincreasedfrequencyofgenemutation.()
答案:对InwhichprocessGG-NERandTC-NERshowdifferent()
答案:DNAlesionrecognitionBaseexcisionrepairisoneofDNArepairpathwaystocorrectslargebaselesions.()
答案:错ThedeficiencyofNERrepairproteinsissusceptibleto_disease.()
答案:XerodermapigmentosumWhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair.()
答案:ThereisnoanyDNAsynthesisWhichofthefollowingDNAdamagesbelongstoexogenousDNAdamage().
答案:IonizingradiationDuringdoublestrandedDNAbreakrepair,NHEJshowsmoreprecisecomparedtoHR.()
答案:错TheOkazakifragmentofeukaryotesislongerthanthatofprokaryotes.()
答案:错ReversetranscriptaseexertsthefunctionsofRNAdependentDNApolymeraseandDNAdependentDNApolymerase.()
答案:对Whichmechanismcanexplaintwostrandsaresynthesizedintheoppositedirection,inthereplicationfork?()
答案:semidiscontinuousreplicationRNAprimerinDNAreplication()
答案:provides3’-OHtosynthesizeDNA;isrequiredbyallOkazakifragmentsWhatarethedifferencesofeukaryoticDNAreplicationfromprokaryotes?()
答案:thereplicationoriginofeukaryotesisshorterthanthatinprokaryotes;eukaryoticDNAcontainsmultipleorigins;eukaryoticDNAisreplicatedinSphaseofcellcycle;telomereandtelomeraseaidterminationofeukaryotesWhichis
FALSEabouttheterminationofprokaryoticDNAreplication?()
答案:CoordinatethesynthesisoflaggingandleadingstrandsAlthough
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