英文医学课件：Lab1 .introduction and epithelium

1、Histology Lab 1 Introduction and Epithelium,Jun zhou（周俊） school of medicine, Zhejiang University,20140922,Lab facilities: 1 Light electron microscope with computer 1 case of slides 1 atlas 1.Students share these facilities 2.Do not take them out the laboratory with you. 3.Put them where they belong

2、to when lab is over. 4.Do not left any trash after you.,Contents of Lab 1: Introduction Epithelium 1)Simple squamous epi (No.1 small intestine, stomach) 2)Simple cuboidal epi.(No.35 thyroid) 3)Simple columnar epi.(No.1 small intestine) (drawing) 4)Pseudostratified ciliated columnar epi.(No.3 trachea

3、) 5)Stratified squamous epi.(No.2 esophagus) 6) Transitional epi.(No.33 bladder) 3.Lab report (content of lab , drawing),Tissue Processing,1.Fixation: 10% Formalin 2.Dehydration:graded concentrated ethyl alcohol 3.Clearing:xylene,infiltrate with paraffin solvent.,Loss of fat due to clearing,Tissue P

4、rocessing,4.Embedding,5.Sectioning,Tissue Processing 6.Staining,Common Stains: Hematoxylin & Eosin,Ross, 5th ed, fig 1.1,Common Stains,Wrights stain,Hematoxylin and eosin (H&E),Relative Sizes in Histology Standard Units of Measure,micrometer: 10-6 meter,nanometer: 10-9 meter,Relative Sizes in Histol

5、ogy Standard Units of Measure,Changes in apparent shape, due to orientation of sections,Ross, 5th ed, fig 1.11,Changes in apparent shape, due to orientation of sections,Netter/Ciba,Junqueira fig 1-30,Epithelium,No blood vessles, nourishment is by diffusion Epithelium is innervated polarization,Trach

6、ea,Epithelium,Classification of Epithelia,Number of cells 1. Simple 2. Pseudostratified 3. Stratified,Shape of surface cells 1. Squamous 2. Cuboidal 3. Columnar 4. Transitional,Stomach (NO 25): mesothelium HE400 Simple squamous epi.,Small intestine (NO 1): endothelium HE400,Thyroid (NO 35): HE100,Th

7、yroid: HE400 Simple cuboidal epi.,Small intestine (NO 1): villus HE400 (Drawing ) Simple columnar epi., Goblet cells, striated border (microvilli),Trachea (NO3): HE100,Trachea: HE400 Pseudostratified ciliated columnar epi.,Esophagus (NO2): HE40 Stratified squamous epi.,Esophagus: HE100,Bladder (NO33): HE 40,Bladder: HE 100,Bladder: HE400 Transitional epi,结缔组织预习:,结缔组织基本特点 分类 LCT铺片如何辨认fibroblast,macrophage, collagen fiber, elastic f