蛋白质试题及答案(河北工程大学).doc

1. dipolarion2. essential amino acid3. (isoelectric point,pI)4. (rare amino acid)5. (nonprotein amino acid)6.(configuration)7.(protein primary structure)8. (conformation)9. (protein secondary structure)10. (domain)11. (protein tertiary structure)12. (hydrogen bond)13. (protein quaternary structure)14. (ionic bond)15. (super-secondary structure)16. (hydrophobic bond)17. ( van der Waals force)18. (salting out)19. (salting in)20. (denaturation)21. (renaturation)22. (precipitation)23. gel electrophoresis24. chromatography()1. _2. _%110mg,_%3. 20_2_.4. _3280nm5. pI10.76,pH7_6. 20_7. _8. -_,_-_9. -_10. _11. _12. _.13. _14. GSH_15. _,_,_16. pH_17. _18. _19. _20. 8.04.510.0,pH8.0_21. pH=pI_pHpI_22. pK1(-COOH)=2.19,pK2 (-NH+3 ) = 9.67,pKR(R)= 4.25,_23. _24. a90 000b45 000c110 000_25. 0.34%_.0.34%4_.26. -180_-_.() 1. pHA. B. C. D. E. 2.A. B. C. D. E. 3.280nmA. B. C. D. 4.4A. B. C. D. 5.A. B. C. D. 6.-A. B. C. 3.6D. 0.15nm.7.A. B. C. D. 8.A. LeuB. AlaC. GlyD. SerE. Val9.A. B. C. D. 10.A. B. C. D. 11.A. pHB. C. D. 12.A. B. C. D. 13.-A. B. C. D. E. 14.-A. -B. C. -D. 0.35nm15.A. B. C. D. 16.A. B. C. D. E. 17.A. B. C. D. 18. A. B. C. D. 19.A. B. C. D. pH20.A. B. C. D. E. 21.A. B. C. ABD. E. 22.A. B. C. D. A,BC() () 1.() 2.() 3.() 4.-() 5.D-L-() 6.() 7.20() 8.() 9.pI-COOH-NH2 () 10.() 11.() 12.() 13.FDNBEdmanN-() 14.20-() 15.R() 16.() 17.() 18.() 19.() 20.() 21.()22.()23. ()24.1. 2. 3. 4. 5. 6. 7. 8. 9. CNBr6mol/L HCl-12344510. 151015Ile-Ala-His-Thr-Tyr-Gly-Pro-Glu-Ala-Ala-Met-Cys-Lys-Try-Glu-Ala-Gln-202527Pro-Asp-Gly-Met-Glu-Cys-Ala-Phe-His-Arg1-231. 2. 3. pHpI4. 205. 6. 7. 8. 9. 10. 11. 12. N-HO-H13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 1. 2.16 6.253.4.5.6.7.-8. C=ON=H0.54nm 3.60.15nm9. 10.11.12.13.14.15.16.17.18.FDNB2,4-Edman19.SDS-SDS-PAGE20.21.22.3.2223.24.Cab25.16 672 66 68726.5027nm1. D59.74,pH2. B83. A280nm540120,215nm,280nm215nm4. D4R3R5. B-6. B-3.60.54nm,0.15nm,B-7. C8. C-429. B-NH10. D11. ApHpH12. A13. E,C=O -;14. C-C-0.35nm, 15. C-16. E22217. A