sequin9.00操作步骤.doc

1. 点击 start new submission，进入以下界面，选择release date,输入需发表文章的标题，点击next page.输入联系方式，点击next page 输入第二、三作者的信息，点击next page,输入单位的信息，点击next form.2.选择environmental samples, 点击next form.3.点击Add/Modify sequences,点击add new sequence再点击import nucleotide FASTA, 打开submission sequences.txt 文件，即把序列已输入，点击save, 回到上一界面。点击Done.3. 点击next page,再点击Add organisms, locations, and genetic codes, 选择uncultured bacterium,再点击左边，点击Accept,回到前一个界面，点击add source modifiersEdit individual values,输入克隆名称，Accept,Apply one value to all, accept,是，Apply one value to all， accept是，Apply one value to all, accept,是。点击OK,Next page, 暂不管PROTEINS, 再next page,选择incomplete at 5 end, 和incomplete at 3 end, 再选中CDS(可翻译成氨基酸序列)，Next form,Annotate ,点击genrate definition line, 可补definition全名。在上面的窗口中可修改definition, organism等信息，只要在修改处双击即可。Annotate ,点击descriptors，可改变前面设定的信息。点击search 菜单中的validate命令，对输入的序列格式进行检查（是否有错误？），之后向数据库提交。本次出现的错误如下图，S61, F15, S68, F86有错误：均出现内部终止密码。用 file 菜单中的prepare submission 将输入的序列及注册信息合成为提交文件，之后后再用email发给各个数据库信箱Genbank: EMBL: datasubebi.ac.ukDDBJ: ddbjsubddbj.nig.ac.jpSequin9.00操作说明邱珊莲2008.11.29Dear GenBank Manager: Here within enclosed is my submission file N10.sqnof nucleotide sequences.The further information about thefile is in the following: 1. including 53 sequences;2. the sequences encode ammonia monooxygenase subunit A；3. each sequence is partial cds. Thank you very much for processing!Sincerely