分离肝癌细胞及其转移性细胞株的建立

Percoll G 20 2 H2M H4M 2 3 71 78 CGH H2M 4q 13q 16q 17p 19p 1q 3q 5p 6p 7q 8q 1q 6p t 1 6 H4M 8p 9 13q 16q 6p 7p 11p 11q13 hsr H2M 1 H4M Percoll 2 hepatocellularcarcinoma HCC 1 2 HCC 1 1 1 20 1 2 1 3 2 3d 2mL 0 25 0 02 TA 10 L 24 1mL 2 2 H2M H4M 1 4 12 H4M 5 4 104 5cm 2 3 7d H2M 16 7 1 04 1 5 H2M H4M 0 3 g ml 3h 0 07mmol L 30min 1 3 2h G 1 6 K SDS H2M H4M DNA DNA CGH 2 1 gH4M DNA DNA dUTP dUTP 500ng DNA DNA 37 48h 0 4 SSC 0 3NP 40 75 2min 2 SSC 0 1NP 40 2min 1 g mLDAPI 1 7 1 8 H2M H4M 12 balb c 9 106 1 2 2 1 20 2 H2M H4M H2M H4M 4 50 2 1 H2M H4M 2 5 47h 38h 3 2 G 2 3 H2M 1q 6 p t 1 6 H4M homogeneouslysta iningregion hsr 2 H4M 71 78 XX H2M 56 78 XY 4 3 CGH H2M 4q 13q 16q 17p 19p 1q 3q 5p 6p 7q 8q H4M 8p 9 13q 16q 6p 7p 11p 11q13 amplification 5 4 H2M balb c 4 2 10mm HE 3 H4M 3 Percoll 3 2 CGH H2M 1q 8q 4q H4M 8p 11q13 4 5 Cheng 6 4 H2M H4M 7 8 H4M 11q13 cyclinD1 CCND1 CyclinD 9 H4M cyclinD1mRNA 10 2 1 2 CHENYB YANML GONGJP etal Establishmentofhepatocellularcarcinomam ultidrugresistantmonoclonecelllineHepG2 mdr1 J ChinMedJ Engl 2 007 20 8 703 707 A 2001 705 749 TEUFELA STAIBF KANZLERS etal Geneticsofhepatocellularcarcinoma J WorldJGastroenterol 2007 28 16 2271 2282 J 2004 25 3S 123 125 ROUGIERP MITRYE BARBAREJC etal Hepatocellularcarcinoma HCC