DNA 条形码会议12PPT课件

精选,ResultsofPastCBOLOutreachMeetings,ScottE.Miller,ChairConsortiumfortheBarcodeofLifeSmithsonianInstitution,精选,UsesofDNABarcodes,Researchtoolforimprovingspecies-leveltaxonomy:Associatingalllifehistorystages,gendersTestingspeciesboundaries,findingnewvariants“Triage”toolforflaggingpotentialnewspecies:UndescribedandcrypticspeciesAppliedtoolforidentifyingregulatedspecies:Diseasevectors,agriculturalpests,invasivesEnvironmentalindicators,protectedspeciesUsingminimalsamples,damagedspecimens,gutcontents,droppings,精选,精选,OutreachActivities,CBOLsregionalbarcodemeetings:Typically6-12countries,60-80participantsResearchers,policy-makers,agencyofficialsPre-meetingsurveysofcapabilities,needsRaiseawarenessofDNAbarcoding,applicationsIdentifypriorities,projects,SteeringCommitteesFollow-onmeetingsforprojectplanning,writinggrantproposalsCo-sponsoredbyBioNET-INTERNATIONALandSwissAgencyforDevelopmentandCooperation(SDC),精选,HistoryofOutreachMeetings,CapeTown,SouthAfrica:April2006,SANBINairobi,Kenya:October2006Campinas,Brazil:March2007Taipei,Taiwan:September2007Abuja,Nigeria:October2008Beijing,China:May2009India,December2009,精选,BarcodingPrioritiesIdentified,PestinsectsinagricultureandquarantineprogramsTreesMedicinalplantsMosquitoesCommercialfishmarineandfreshwaterOrnamentalfishintradeEndangeredmammalsReptilesandamphibiansEndangeredspeciesininternationaltrade,精选,CriticalElementsforImplementation,CommittedleaderwillingtomanageParticipatingcountriesmusthavecommongoalsBalancedbutsmallSteeringCommitteeRepresentativeTaxonomicexpertsMinistry/AgencyofficialsPotentialfundingsourcesidentifiedearlyRealisticgoalsandtimetableFull-timetechnicianorpost-docneededtomakesignificantprogress,精选,OtherCBOLInitiatives,InadditiontoRegionalOutreachMeetings,CBOLorganizesmajorCampaignsandDemonstratorProjects:FISH-BOLAll-BirdsBarcodingInitiativeMarineBarcodingInitiativeMosquitoandTephritidBarcodingInitiativesWaterQualityBioassessmentAll-FungalBarcodingBeeBarcodingInitiative,精选,ProjectPlanningMeetings,Includetaxonomists,users,partnersforlabandinformaticsservicesDiscussgoals:basicresearch,applications?ReviewcurrentbarcodingandtaxonomicworkResolvelogisticalissuesEstimateneeds,timeline,andbudgetDivisionoflabor,includingproposalwritingTargetsforfunding,精选,LaunchingBarcodingProjects,AssemblingSteeringCommitteeUsersTaxonomists,collectioncuratorsServiceproviders(BOLD,analyticallabs)Planforscope,timetable,logisticsPilottestsofprimers,PCRamplificationOrganizesupplyofspecimenstolabAddressotherlogisticalissues,精选,LogisticalIssues,Organization:Bytaxonomy?Geography?Application?TaxonomicFramework:ConsensuslistofspeciesSamplingStrategySupplyofsamplesAssemblingandcomparingdataFundingResultingPublications,精选,SamplingStrategy,Howmanyspecimensperspeciesperarea?UnderstudybyDataAnalysisWorkingGroupFISH-BOL,ABBI:5perspeciesfromacrossgeographicrangeMinimumonewholevoucherperspeciesIntensivesamplingforsomespeciesWhatqualifiesasavoucher?Varieswidely:Goldstandardmuseum/herbariumstudyspecimensInvasivenon-destructiveextractionsamplingTotallydestructivesamplingwithe-Voucher,精选,SupplyofSpecimens,Sourcesofspecimens:Museumcollections()Piggybackonexistingfieldprograms()Dedicatednewcollectingefforts()Permitsforcollecting/internationaltransferExpertidentificationDatacapture,digitalimaging,tissuesamplingCurationofvoucherspecimens,tissuesamples,DNAextractsMovingsamples(orextractsorPCRproducts)tosequencers,精选,DataManagement,AssemblingandcomparingdataCBOLrecommendsBOLDwithsubsequentuploadtoINSDC(GenBank,DDBJ,EMBL)CurationandqualitycontrolCBOL/INSDChaveestablishedstandards,capabilitytomaintaindataaccuracyLong-termresponsibilityofbarcodingcommunity,精选,ReleaseofData,Ownership,pre-releaseaccessProject-specificpoliciesAttitudesrangefromveryopentomoreprotectiveTimingofdatareleaseControlledbyownerofdataCBOLurgesearlyrelease,exceptfortightconnectionstohypothesis-drivenpublications,精选,ResultingPublications,PilotstudiesdemonstratingeffectivenessofbarcoderegioninanewtaxonRegionalbarcodesurveysofataxon,notingtrendstowardlumpingorsplittingUseofbarcodesinecological,evolutionarystudies,appliedprojectsProgressreportsfrombarcodingcampaignsSyntheticstudiesofregions,habitats,taxaFormaltaxonomicrevisions,精选,Specimens,Characters,DistributionsofCharacterVariation,TaxonomicDecision-Making,SocioeconomicDecisions,Specimens,Concerns/Regulations,ThePracticeofTaxonomy,Taxonomists,TheUsesofTaxonomy,精选,AdoptionbyRegulators,USAFoodandDrugAdministrationReferencebarcodesforcommercialfishUSANOAA/NMFS$100KforGulfofMainepilotprojectFISH-BOLworkshopwithagencies,Taipei,Sept2007USAFederalAviationAdministration$500KforbirdsUSAEnvironmentalProtectionAgency$250Kpilottest,waterqualitybioassessmentwithinsectsFAOandInternationalPlantProtectionConventionProposalforDiagnosticProtocolsforfruitfliesCITES,NationalAgencies,ConservationNGOsInternationalSteeringCommittee,identifyingpilotprojects,DNABarcodingApplied:BirdstrikeIdentification,Eachyearbird-aircraftcollisions(birdstrikes)causeanaverageof$44Mindamagestomilitary,and$354Mindamagestocivilaircraft.,DNABARCODINGAPPLIED,精选,FisheriesApplications,ImprovingstockassessmentsLarvalfishSpot-checkingbycatchBettertaxonomyanddistributionmapsFoodchainanalysisfromgutcontentsValidationofimportspeciesFiletsCaviarProcessedproductsBallastwater,精选,AssociatingLifeStages,ProcessedParts,DimorphicGenders,精选,Fisheries:mislabellingofadepletedreeffishNATURE2004Jul15;430(6997):309-10MarkoPB,LeeSC,RiceAM,GramlingJM,FitzhenryTM,McAlisterJS,HarperGR,MoranAL.Anyfishspeciesthatappearstobereadilyavailableinthemarketp