酶切位点的保护碱基原则

1、1 bp 2bp3bp4bp5bpAciI- + + + + AgeI+ + + + + AgeI-HF+ + + + + AluI- + + + + ApaI+ + + + + AscI+ + + + + AvrII+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpBamHI+ + + + + BamHI-HF®+ + + + + BglII+ + + + + BmtI+ + + + + BmtI-HF®+ + + + + BsaI+ + + + + BsaI-HF®+ + +

2、+ + BsiWI+ + + + + BsmBI+ + + + + BsrGI+ + + + + BssHII+ + + + +1 bp 2bp 3bp4bp5 bpClaI- - + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpDdeI+ + + + + DpnI- + + nt nt DraIII+ + + + + DraIII-HF®+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpEagI+ + + + + EagI

3、-HF®+ + + + + EcoRI+ + + + + EcoRI-HF®+ + + + + EcoRV+ + + + + EcoRV-HF®+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpFseI+ + + + + Enzyme Back to top Base Pairs from end1 bp2345 bpbp bp bpHindIII- + + + + HindIII-HF®- + + + + HpaI+ + + + + Enzyme Back to top

4、 Base Pairs from end1 bp 2bp 3bp4bp5 bpKpnI+ + + + + KpnI-HF®+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpMfeI+ + + + + MfeI-HF®+ + + + + MluI+ + + + + MseI+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpNcoI- + + + + NcoI-HF®+ + + + + NdeI+ +

5、+ + + NheI+ + + + + NheI-HF®+ + + + + NlaIII+ + + + +NotI-HF®+ + + + + NsiI+ + + + + NspI- - + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpPacI+ + + + + PciI+ + + + + PmeI+ + + + + PstI+ + + + + PstI-HF®+ + + + + PvuI+ + + + + PvuI-HF®+ + + + + PvuII+ + + + + Pvu

6、II-HF®- + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpRsaI+ + + + + Enzyme Back to top Base Pairs from end1 bp 2bp 3bp4bp5 bpSacI- + + + + SacI-HF®- + + + +SalI- + + + + SalI-HF®- + + + + SapI+ + + + + Sau3AI+ + + + + SbfI+ + + + + SbfI-HF®+ + + + + ScaI+ + + + + ScaI-HF®+ + + + + SfiI+ + + + + SmaI+ + + + + SpeI+ + + + + SpeI-HF®+ + + + + SphI-HF®+ + + + + SphI+ + + + + SspI+ + + + + SspI-HF®+ + + + + StuI+ + + + + StyI+ + + + + StyI-HF®+ +