生物工艺学教学课件：Chapter 07 Scale-up of Aerobic Processes

1、Chapter 7Scale-up of Aerobic Processes7.1. Introduction of Scale-up反应器内的浓度场、温度场、速度场分布颗粒团聚、气泡聚并颗粒聚团内的菌体细胞细胞内代谢网络基因DNA、RNA网络ReactorCell metabilismGene express 放大过程基于细胞群体效应和传递特性的直接放大新思路 摇瓶实验室发酵罐工业化生产中试生产面向：传统的生物过程逐级放大方法Scale up(1) inherent propertyGene influecnce metabilism (2)EnvironmentalMass trasfor

2、mationHomogenious system7.2. Power Calculations 7.1. Single stirrer in anaerated medium 7.2. Mutile stirrer in anaerated medium 7.3. Calculation of KLa Step by step scale up Mathmatical method Scale up criteria P/V Pg/V KLa tip rate mixing time 7.4. Scale-up CriteriaSize scale up7.4.1Scale up of siz

3、e of reactor Constant of air rate per volume（VVM1）（VVM2）（状态）7.4.2Scale up of air input Constant of air rate per square area Constant of KLacorrection：改为：代入：VVMWgScale up methodbeforeafterbeforeafterVVm constant1113.33Wg constant10.311KLa constant10.51811.71 P/ Vconst 7.4.3 Scale up of power and spee

4、d of stirrer Pg/V=constant KLa =constant Stirrer reactor若 0.95 0.67 air lift reactorWtV power per volume Vtip=constantNRe P/V =constantT D diameter of tank and stirrer power per volume Tmix=constantConstant parameterStirrer speed in 10m3 vesselPower per volume (Pg/V)85Oxygen transfer (KLa)79Shear ra

5、te (NDi)50Mixing time (tm)1260Stirrer speed requirement when scaling up from a 10L STR to a 10m3 fermenter using different criteria7.5. Microbial responses to concentration gradients3.7L罐自制栅式桨 1500L罐特制搅拌桨 摇瓶发酵罐模拟计算平台流场测试与分析工业化生产创新思路基于细胞群体效应和传递特性的直接放大新思路 信号分子发酵罐内流场分布发酵罐内空气分布1mm3mm5mm8mm10mm不同直径的气泡分布不同转速和通气量条件下流场的比较90rpm，1.5VVM105rpm，1VVM1mm90rpm，1.5VVM105rp