Trigonelline-chloride-Trigonelline-hydrochloride-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETrigonelline chlorideCat. No.: HY-N0415CAS No.: 6138-41-6Synonyms: Trigonelline hydrochloride分式: CHClNO分量: 173.6作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 6 mg/mL

2、 (34.56 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 5.7604 mL 28.8018 mL 57.6037 mL5 mM 1.1521 mL 5.7604 mL 11.5207 mL10 mM 0.5760 mL 2.8802 mL 5.7604 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Trigonelline chloride种量存在于咖啡中并且具有潜在抗糖尿病活性的物碱。体外研究It i

3、s found that Trigonelline chloride (TG) significantly rescues the morphology of the H9c2 cells. Treatment ofcells with Trigonelline chloride attenuates H2O2 induced cell deaths and improves the antioxidant activity. Inaddition, Trigonelline chloride regulates the apoptotic gene caspase-3, caspase-9

4、and anti-apoptotic geneBcl-2, Bcl-XL during H2O2 induced oxidative stress in H9c2 cells. For evident, flow cytometer results also1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEconfirm that Trigonelline chloride significantly reduces the H2O2 induced necrosis and apoptosis in H9c2cells. However, fu

5、rther increment of Trigonelline chloride concentration against H2O2 can induce thenecrosis and apoptosis along with H2O2 1.体内研究 Trigonelline chloride decreases bone mineralization and tends to worsen bone mechanical properties instreptozotocin-induced diabetic rats. In nicotinamide/streptozotocin-tr

6、eated rats, Trigonelline chloridesignificantly increases bone mineral density (BMD) and tends to improve cancellous bone strength.Trigonelline chloride differentially affects the skeletal system of rats with streptozotocin-induced metabolicdisorders, intensifying the osteoporotic changes in streptoz

7、otocin-treated rats and favorably affecting bonesin the non-hyperglycemic (nicotinamide/streptozotocin-treated) rats 2.PROTOCOLCell Assay 1 The H9c2 cells are seeded in the 96 well at a density of 1105 cells/well. The cells are treated with differentconcentrations of Trigonelline chloride (TG) (25 t

8、o 150 M) and hydrogen peroxide (25 to 125 M). It isincubated at 37C in 5% CO2 incubator for 24 h and 6 h respectively and then the culture is treated with thewater soluble tetrazolium (WST) reagent incubated for 2 h to 4 h. The living cells absorb the WST then it isconverted into an orange colour pr

9、oduct. Then, the intensity of colour is measured at 450 nm using spectracount ELISA reader. For cardio protective activity, the cells are seeded and separated into six groups:control, H2O2 alone, the rest of groups are initially exposed to different concentration (25 to 125 M) ofTrigonelline chlorid

10、e for 48 hours. Then, 100 M of H2O2 is added and incubated for 4 hours, after, read theabsorbance at 450 nm for cell viability assay 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Three-month-old female Wistar rats are used in this study. The

11、animals are divided into five groups (n=10):Administration 2 Control rats, Streptozotocin-treated control rats, Streptozotocin-treated rats receiving Trigonelline chloride(50 mg/kg p.o. daily), Nicotinamide/streptozotocin-treated control rats, and Nicotinamide/streptozotocin-treated rats receiving T

12、rigonelline chloride (50 mg/kg p.o. daily). Administration of Trigonelline chloride startstwo weeks after streptozotocin and lasts four weeks. Trigonelline chloride is administered once daily by astomach tube. All control rats receive tap water (the vehicle) at the same volume of 2 mL/kg p.o. The fo

13、ur-week period of Trigonelline chloride administration is long enough to demonstrate skeletal effects ofTrigonelline chloride and other compounds of plant origin in rats. The rats are fasted overnight after the lastTrigonelline chloride or vehicle administration. The next day, the blood glucose leve

14、l is measured and therats are anesthetized with ketamine and xylazine, and then sacrificed by cardiac exsanguination 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Ilavenil S, et al. Trigonelline protects the cardiocyte from hydrogen peroxide induced apoptosis in H9c2 cells. Asian Pac J Trop Med.2015 Apr;8(4):263-8.2. Joanna Folwarczna, et al. Effects of Trigonelline, an Alkaloid Present in Coffee, on Diabetes-Induced Disorders in the Rat SkeletalSystem. Nutrients. 2016 Mar; 8(3): 133.McePdfHeight2/2 Master of