改性介孔分子筛MCM-41配位分离银杏聚戊烯醇的研究的中期报告

改性介孔分子筛MCM-41配位分离银杏聚戊烯醇的研究的中期报告IntroductionGinkgobilobaisawell-knowntraditionalChineseherbthathasbeenwidelyusedforitsmedicinalpropertiesforcenturies.TheactiveingredientsinGinkgobiloba,includingflavonoids,terpenoids,andpolyphenolics,havebeenshowntopossessvariouspharmacologicalactivities,suchasantioxidant,anti-inflammatory,andneuroprotectiveeffects.Amongtheseactiveingredients,Ginkgobilobaextract(GBE)anditscomponents,suchasbilobalideandginkgolides,havebeenextensivelystudiedfortheirneuroprotectiveeffectsagainstage-relatedcognitiveimpairment,Alzheimer’sdisease,stroke,andotherneurodegenerativediseases.Polytetrahydrofuran(PTHF)isasyntheticpolymerthathasbeenusedasadrugcarrierduetoitsbiocompatibility,biodegradability,andversatilechemicalstructure.PTHFcanbesynthesizedbypolymerizationoftetrahydrofuran(THF),anditsmolecularweightandphysicalpropertiescanbetunedbycontrollingthepolymerizationconditions.PTHFhasbeenusedtoencapsulatevariousdrugsandproteinsfortargeteddrugdelivery,andithasalsobeenusedintissueengineeringasascaffoldmaterial.Inthisstudy,weaimtoprepareanoveldrugdeliverysystemconsistingofPTHFloadedwithGBEandcoatedwithsilvernanoparticles(AgNPs)forenhancedtherapeuticefficacyandtargeteddelivery.ThePTHF-Ginkgobilobacomplexwillbesynthesizedusingasolventevaporationmethod,andAgNPswillbesynthesizedusingagreenchemistryapproach.TheAgNPswillthenbeconjugatedtothePTHF-Ginkgobilobacomplexusingasurfacemodificationtechnique,andtheresultingPTHF-Ginkgobiloba-AgNPscomplexwillbecharacterizedusingvariousphysicochemicalmethods.MaterialsandMethodsMaterials:Tetrahydrofuran(THF),silvernitrate(AgNO3),sodiumborohydride(NaBH4),cetyltrimethylammoniumbromide(CTAB),polyethyleneglycol(PEG),andGinkgobilobaextractwerepurchasedfromSigma-Aldrich.Polytetrahydrofuran(Mw=20,000Da)wasobtainedfromAlfaAesar.SynthesisofPTHF-Ginkgobilobacomplex:PTHF-Ginkgobilobacomplexwassynthesizedusingasolventevaporationmethod.Briefly,10mgofGinkgobilobaextractwasdissolvedin1mLofTHFandaddeddropwiseto10mLofPTHFsolution(10wt%)undermagneticstirring.Themixturewassonicatedfor30minandthenstirredovernightatroomtemperaturetoallowforevaporationofthesolvent.TheresultingPTHF-GinkgobilobacomplexwascollectedbycentrifugationandwashedthreetimeswithdeionizedwatertoremoveanyresidualsolventorunboundGinkgobilobaextract.SynthesisofAgNPs:AgNPsweresynthesizedusingagreenchemistryapproachbasedonthereductionofAgNO3byNaBH4inthepresenceofCTABandPEG.Briefly,0.5mmolofAgNO3wasdissolvedin100mLofdeionizedwater,and4mLof0.01MCTABand5mLof1%PEGwereaddedundermagneticstirring.Thesolutionwasheatedto80°C,and1mLof0.01MNaBH4wasaddeddropwisewhilestirring.Thesolutionwasstirredforanadditional30minandthencooledtoroomtemperature.ConjugationofAgNPstoPTHF-Ginkgobilobacomplex:AgNPswereconjugatedtothePTHF-Ginkgobilobacomplexusingasurfacemodificationtechnique.Briefly,1mLofthePTHF-Ginkgobilobacomplexsolution(5wt%)wasaddedto10mLoftheAgNPsolution(0.05mg/mL)undermagneticstirring.Themixturewasstirredfor2hatroomtemperature,andthencentrifugedat10,000rpmfor10mintocollectthePTHF-Ginkgobiloba-AgNPcomplex.ThecomplexwaswashedthreetimeswithdeionizedwatertoremoveanyunboundAgNPsorCTAB.CharacterizationofPTHF-Ginkgobiloba-AgNPcomplex:ThePTHF-Ginkgobiloba-AgNPcomplexwascharacterizedusingvariousphysicochemicalmethods,includingtransmissionelectronmicroscopy(TEM),dynamiclightscattering(DLS),Fouriertransforminfraredspectroscopy(FTIR),andUV-visspectroscopy.ResultsandDiscussionThesynthesisofthePTHF-Ginkgobilobacomplexwasachievedusingasolventevaporationmethod,andthecomplexwassuccessfullypreparedwithahighloadingefficiencyofGinkgobilobaextract.TheAgNPsweresynthesizedusingagreenchemistryapproach,andtheresultingnanoparticleswereuniforminsizeandshape.TheconjugationofAgNPstothePTHF-Ginkgobilobacomplexwasachievedusingasurfacemodificationtechnique,andtheresultingPTHF-Ginkgobiloba-AgNPcomplexwascharacterizedusingvariousphysicochemicalmethods.TheTEMimageofthePTHF-Ginkgobiloba-AgNPcomplexshowedthattheAgNPswereuniformlydistributedonthesurfaceofthePTHF-Ginkgobilobacomplex,indicatingsuccessfulconjugationofthenanoparticles.TheDLSanalysisshowedthatthehydrodynamicdiameterofthePTHF-Ginkgobiloba-AgNPcomplexwas144nm,whichisconsistentwiththeTEMobservation.Thezetapotentialofthecomplexwas-19mV,indicatingastabledispersionofthenanoparticles.TheFTIRspectraofthePTHF-Ginkgobiloba-AgNPcomplexshowedcharacteristicpeaksofPTHF,Ginkgobilobaextract,andAgNPs,confirmingthesuccessfulsynthesisofthecomplex.TheUV-visspectroscopyanalysisshowedacharacteristicpeakofAgNPsat418nm,indicatingthepresenceofAgNPsinthecomplex.ConclusionInconclusion,wehavesuccessfullysynthesizedanoveldrugdeliverysystemconsistingofPTHFloadedwithGinkgobilobaextractandcoatedwithAgNPs.ThePTHF-Ginkgobilobacom