雨课堂学堂在线学堂云《Food Microbiology Testing（华南农大 ）》单元测试考核答案

注：不含主观题第1题单选题(7分)Refrigeratedtemperatureis（）AthetemperaturebetweenroomtemperatureandrefrigeratingtemperatureB0-5℃Ctemperaturebelow0℃Dtemperaturebelow-20℃第2题单选题(7分)Theamountofagaraddedtothesolidmediumwas（）A0.01B0.02C0.03D0.05第3题单选题(7分)High-sugarmediumisusedtocultivateyeast.Themediumtypeis（）Aenriched

medium

BselectivemediumCdifferentialmediumDnormalmedium第4题单选题(7分)Substancesthatarenotgrowthfactorsare（）Aamino

acidBmineralsCpurinebaseDvitamin第5题单选题(7分)Thebestcarbonsourceformostheterotrophicmicroorganismsis（）Aglucose

Borganic

acidCalcohol

Dlipid

第6题单选题(7分)Theoptimumconcentrationofalcoholfordisinfectionis（）A0.5B0.65C0.75D0.95第7题单选题(7分)Pasteurization,commonlyusedinthefoodandbeverageindustry,isa()method.AdisinfectionBbacteriostasisCdisinfectionDdegerming第8题单选题(7分)Inthegrowthcurveofbacteria,thetotalnumberofbacteriaandthenumberofviablebacteriaarealmostequalin（）AadaptivephaseBlogarithmic

phase

CstationaryphaseDdecline

phase

第9题判断题(7分)Whencultivatingmicroorganisms,carbonandnitrogensourcesmustbeaddedmanually.第10题判断题(7分)Thethreebasicformsofbacteriaarespherical,rodandspiral.第11题多选题(15分)Accordingtothestateofthemediumaftersterilizationandafterreturningtoroomtemperature,themediumcanbedividedinto（）A

fluid

mediumBsolid

mediumCsemisolid

mediumDplatingmedium正确答案：ABC第12题多选题(15分)Thecommonmicrobialinoculationtechniquesare（）AplateplantingmethodBsolidbevelpunctureinoculationCsemi-solidpunctureinoculationDinoculationliquidmedium正确答案：ABCDChapter3exercises第1题单选题(7分)Whichvalueisnotsetinthesecondarysamplingscheme？AMBnCcDm第2题单选题(7分)Whichofthefollowingbacterianeedstoadoptasecondarysamplingscheme？AEscherichiacoliBVibrio

parahemolyticusCStaphylococcus

aureus

DEnterobacter

sakazakii第3题单选题(7分)Whichofthefollowingbacteriadoesnotrequireasecond-levelsamplingscheme？AClostridium

botulinumBListeriamonocytogenesCStaphylococcus

aureus

DEnterobacter

sakazakii第4题单选题(7分)Amongtheprecautionsforsedimentationbacteriasampling,theerroris（）Athesedimentationbacteriasamplingpointshouldbemorethan1mawayfromthewallBtheheightofthesamplingpointisinprincipleconsistentwiththeheightofthehumanbreathingzone,andtherelativeheightisbetween0.5-1.5mCtustunderstandtheenterprisecommonlyuseddisinfectanttypecharacteristicsinadvanceDsamplingpointsshouldbeavoidedfromvents第5题单选题(7分)Whichofthefollowingstatementsistrue？Aonlyincasesofserioushazardshouldasecond-levelsamplingschemebeusedBthedetectionofhygienicindicatorbacteriacanbedetectedinthe

BSL-1.CthelayoutofthemicrobiologylaboratoryshouldadoptamultidirectionworkflowDforsamplingofproductionwater,fine-mouthedbottleswithdust-proofandcorkedbottlestoppersshouldbeused第6题单选题(7分)Thelayoutofthemicrobiologylaboratoryshouldadopta（）workflowtoavoidcross-contaminationAone

directionBmultidirectionCleft-rightdirectionDup-down

direction

第7题单选题(7分)Thefollowingwhichisnotasolidsampleprocessingmethodis（）.AshearingandshakingmethodBgrindingmethodCwhole-grainshakingmethodDdirectprocessingmethod第8题判断题(7分)Accordingtothetypesofmicrobialhazards,formicrobeswithmoderateorevenseverehazards,athree-levelsamplingschemeshouldbeusedforsamplesampling;forlow-levelhealthhazards,asecond-levelsamplingschemeisrecommended.第9题判断题(7分)Thedetectionofhygienicindicatorbacteriacanbedetectedinthe

BSL-1.Theisolationandidentificationofpathogenicmicroorganismsshouldbedetectedin

BSL-3laboratory

orabove.第10题判断题(7分)Thecollectionofsamplesshouldfollowtheprincipleswhichareplanningandrepresentativeness.Thesamplingprocessfollowsasepticprocedurestopreventallpossibleexternalcontamination.第11题多选题(15分)Whichofthefollowingrequirementsshouldfood

inspectorsmeet?AnormalcolorvisionBhavethecorrespondingmicrobiologyprofessionaleducationCshouldhavethecorrespondingqualificationtobeabletounderstandandimplementtheinspectioncorrectlyDmastertheknowledgeoflaboratorybiosafetyoperationanddisinfection正确答案：ABCD第12题多选题(15分)Samplingofliquidsamplesrequiresthefollowingsteps？（）AshakewellBsurfacedisinfectionCopeningwithsteriletoolsDpipettesamplesbelow2.5cmfromtheliquidsurface正确答案：ABCDChapter4exercises第1题单选题(7分)AccordingtoGB4789.2－2016Detectionofaerobicplatecount,whentheliquidsampleismeasured,ifbothofthepetridishesinoculatedwiththestocksolutionappearasepticallydropped,itshouldreport()CFU/ml.A<1B<10C<1×dilutionfactor第2题单选题(7分)The()fermentationtestisofgreatsignificanceinthemicrobiologicalexaminationofColiformbacteriainfood.AglucoseBlactoseCmaltoseDmannitol

第3题单选题(7分)Inenvironmentalhealth,drinkingwaterhygieneandfoodhygiene,thecommonlyusedindexfordetectionoffecalcontaminationis().AStreptococcus

faeciumBColiformCSalmonellaDStaphylococcus

aureus

第4题单选题(7分)Themostaccuratemethodofviablecountis().AdirectmicroscopiccountBMPNCpourplatecountingmethodDrapidcolonycounting第5题单选题(7分)ThenationalcountstandardforE.coliis().AGB4789-2010BGB4789.3-2016CGB4789.3-2008DGB/T4789.3-2003第6题单选题(7分)Chineseurbandrinkingwaterhygienestandardsstipulatethat().AthetotalnumberofbacteriapermLofwaterislessthan1000BthetotalnumberofbacteriapermLofwaterislessthan100CthetotalnumberofbacteriapermLofwaterislessthan10Dthetotalnumberofbacteriaper500mLofwaterislessthan1000第7题单选题(7分)Whentestingintestinalbacteria,thecultureshouldbeincubatedat().A28℃B25℃C37℃D42℃第8题判断题(7分)Whencountingcolonies,ifthenumberofplatecoloniesofalldilutionsislessthan30CFU,thentheaveragenumberofcolonieswiththelowestdilutionshouldbemultipliedbythedilutionfactor第9题判断题(7分)Forthepreparationof10-foldseriesdilutedsamplehomogenate,thesterilepipettesorsuctionheadshouldbechangedonceforeachincrementaldilution.第10题判断题(7分)Theresultsobtainedfromdetectionofaerobicplatecountcanfullyreflectthebacterialcontaminationofcontaminatedfood.第11题多选题(15分)TherearethreefoodmicrobiologicalindexesissuedbytheMinistryofHealthinChina:（）AaerobicplatecountBColiformCEscherichiacoliDpathogenic

bacterium正确答案：ABC第12题多选题(15分)WhichofthefollowingisfalseaboutthedetectionofColiform？()AMPNmethodistocalculatethemaximumpossiblenumberofescherichiacoliinthesamplestobetestedbystatisticalprobabilitytheorybasedonthelowestdegreeofungrownandthehighestdegreeofgrowthdilutionofthesamplestobetestedafterserialdilutionandcultureBinMPNmethod,thewholeprocessfromsamplepreparationtosampleinoculationshouldnotexceed20minCintheprimaryfermentationexperiment,thenon-gas-producingsubjectswereculturedfor24±2hfortherefermentationexperimentDanyBGLBbrothtubethatproducesgascanbereportedpositiveforthatmicroflora正确答案：BCChapter5exercises第1题单选题(6分)Thefollowingpre-enrichmentsolutioncanbeusedforpre-enrichmentofSalmonellais().AdextrosebrothBbufferpeptonewaterCsodiumchloridebroth,7.5%D

nutrient

broth第2题单选题(6分)WhichofthefollowingtypesofmicrobesisSalmonella?()AaerobicBoxygen-resistantCaerobicorfacultativeanaerobictypeDanaerobic第3题单选题(6分)WhatkindofculturemediumisSalmonellacultured?Itscolonyischaracterizedbyblackcolonywithmetallicluster,tanorgray,andtheculturemediumaroundthecolonyisblackorbrown.()ABSAgarBHEAgarCXLDAgarDSalmonellachromogenicmedium第4题单选题(6分)The()isacomponentofthecellwallofSalmonellaandisgranularinitsresponsetoantiserum.AO-antigenBH-antigenCVi-antigenDK-antigen第5题单选题(6分)ThemainsourceofSalmonellais().AairBmarine

products

Cdomesticanimalanddomestic

fowlDcereals

第6题单选题(6分)TheChineseinspectionstandardforSalmonellais().AGB4789.4-2003BGB4789.4-2010CGB4789.4-2012DGB4789.4-2016第7题判断题(6分)Inthepre-enrichmentstepofSalmonella,bufferedpeptonewater(BPW)doesnotcontaininhibitoryingredients,whichisbeneficialtorepairdamagedcellstoastablebiochemicalstate.第8题判断题(6分)Salmonellaisnotheat-resistantandcanbekilledat55℃for1hor60℃for15-30min.第9题判断题(6分)Salmonelladoesnotproduceindolesanddoesnotbreakdownurea,whichisnegativeinVPtests,andmostproducehydrogensulfide.第10题多选题(11.5分)InGB/T4789.4-2016DetectionofSalmonella,themediausedfortheselectivescreeningofSalmonellaare().AXLDBSalmonellachromogenicmediumCHEDBS正确答案：ABCD第11题多选题(11.5分)Theisolatedtestbacteriaappeared()onTripleSugarIronAgar,indicatingthatthebacteriaweresuspectedSalmonella.Aslantred,bottomyellow,gasproduction,productionofhydrogensulfideBslantred,bottomyellow,nogas,productionofhydrogensulfideCslantred,bottomyellow,nogas,nohydrogensulfideDslantred,bottomred,nogas,nohydrogensulfide正确答案：ABC第12题多选题(11.5分)ThemaintypesofhumanSalmonellainfectionare().AtyphimuriumBsepticemiaCgastroenteritisDcholeraic正确答案：ABCD第13题多选题(11.5分)ThebiochemicalidentificationofSalmonellaincludes().AlysinedecarboxylasetestBKCNtestCindoletestDpH7.2ureatest正确答案：ABCDChapter6exercises第1题单选题(6分)RegardingthedetectionofStaphylococcusaureus，notincluded().Aidentificationexperiments,includingdynamicexperimentsBusebaird-parkerplatetoseparateCgrampositiveDtheplasmacoagulasetestwaspositive第2题单选题(6分)Thepathogenthatproducesplasmacoagulaseis().A

Salmonella

BListeriamonocytogenesCEscherichiacoliDStaphylococcus

aureus

第3题单选题(6分)ThecharacteristicofS.aureuscolonyonbloodplateis().Agoldenyellow,largeroundraisedcoloniesBgoldenyellow,smooth-surfacedroundcoloniessurroundedbyhemolyticringsCgoldenyellow,transparent,largeround,raisedcolonyDwhitetransparentroundcolony第4题单选题(6分)ThefollowingdescriptionofStaphylococcusaureusistrue:()AgramnegativeBplasmacoagulaseisproducedCspecialmediumisrequiredforcultureDpoorsaltresistance第5题单选题(6分)ThesymptomsofStaphylococcusaureusinfectionare().Apusthick,yellow,noodourBthepusisthin,largeandreddishCthepusispalegreenwithaspecialsweet,fishyodorDthepushasapeculiarfoulodor第6题单选题(6分)ThefollowingdescriptionofStaphylococcusaureusistrue:()AthegramstainisredB

nocapsuleCcanproducesporesDdoesnotdecomposelactoseandmannose第7题单选题(6分)TheenrichmentsolutionusedtodetectStaphylococcusaureusis().Asodiumchloridebroth,7.5%BroutinebrothCpeptonewaterDTTB第8题判断题(6分)Staphylococcusaureuscancausepurulentinflammation,alsoknownaspurulentcocci.Inaddition,Staphylococcusaureusalsosecretesmanytoxins,whichisoneofthecausesoffoodbornediseases第9题判断题(6分)Staphylococcusaureusiseasytoculture,haslownutritionrequirements,andisfacultativeanaerobic第10题判断题(6分)Staphylococcusaureuscandecomposeglucose,butnotmaltose第11题判断题(6分)AfewstrainsofStaphylococcusaureushavecapsularmucousmaterialontheouterlayerofthecellwall,whichcanlyseorbecomeL-shapedundertheactionofcertainchemicals.第12题判断题(6分)Samplescollectedbyfoodmicrobiologytestingcanbedisposedofatanytimeafterthecompletionoftesting.第13题判断题(6分)Thecollectedfoodpoisoningsamplesshouldberefrigeratedwhensubmittedforinspection,andpreservativescanbeaddedifnecessary.第14题多选题(11分)（）appearhemolysisonbloodplate.AShigellaBStaphylococcus

aureus

CListeriamonocytogenesD

Salmonella

正确答案：BC第15题多选题(11分)ThefollowingdescriptionofStaphylococcusaureusistrue:()AcansurviveinthehyperosmoticenvironmentBnoteasytoproducedrugresistance,strongresistanceCacompletelytransparenthemolysisringisformedonthebloodplateDgram-positivecoccus正确答案：ACDChapter7exercises第1题单选题(7分)Whichofthefollowingmediumisusedfortheenumerationofmouldsandyeasts?()AblondagarBeosin-methyleneblueagar

CcookerosebengalagarDnutrientagar第2题单选题(7分)Theappropriaterangeformouldsandyeastscountis().A30CFU-300CFUB10CFU-150CFUC15CFU-150CFUD10CFU-300CFU第3题单选题(7分)Inthedetectionofmouldsandyeastsinfood,thereasonforaddingchloramphenicolorpenicillintothepotatoglucosemediumis().AgrowthpromotingfactorBpHbufferCinhibitbacterialgrowthDseparatingmouldsandyeasts第4题单选题(7分)Ingeneral,theoptimalculturetemperatureofbacteriais36℃±1℃,andthemouldsis().A26℃±1℃B28℃±1℃C30℃±1℃D32℃±1℃第5题单选题(7分)()ispreferredfortheseparationofmouldsandyeastsfrombeverage.AsaltczapekdoxagarBpotatomediumCcookerosebengalagarDpotato

dextrose

agar

第6题判断题(7分)mouldsandyeastsarebotheukaryotes,andbacteriaareprokaryotes第7题判断题(7分)mouldsandyeastsarebothfungi,andtheirstructuresarebasicallysimilar.第8题判断题(7分)Themostcommonlyusedmethodforcountingbacteria,mouldsandyeastsistheplatemethod.第9题判断题(7分)Beefextractpeptonemediumissuitableforseparatingandcultivatingyeastsandmoulds.第10题判断题(7分)Thesamplesolutionwaspouredintopotatodextroseagar.Aftertheagarsolidified,theplatewasinvertedandculturedina36±1℃incubator.第11题多选题(15分)Accordingto"GB4789.15－2016Enumerationofmouldsandyeasts",225ml()diluentcanbeusedforsampledilution.Onedilutiondegreeofsamplediluentwasaddedto1mleachintwoPetridishes.AdistillatedwaterBnormal

salineCPBSDpeptone

water正确答案：ABC第12题多选题(15分)Accordingto"GB4789.15－2016Enumerationofmouldsandyeasts",methodsfordetectingmouldsandyeastare（）.AmouldsandyeastplatecountmethodBmouldscountedbydirectmicroscopicexaminationCMPNmethodDchromogenicmediummethod正确答案：ABChapter8exercises第1题单选题(5分)Whatisthevaluerepresentedby"thetimerequiredtokill90%oftheoriginalviablebacteriacountunderacertainthermallethaltemperature"?（）AZvalueBDvalueCFvalueDODvalue第2题单选题(5分)ThepHoflowacidcannedfoodis().ApH<3.7BpH3.7~4.6CpH4.6~5.0DpH>5.0第3题单选题(5分)Whichofthecommontypesofcannedbulgesiswrong?()AphysicalbulgingBchemical

bulgingCbacterialbulgingDenvironmental

pollution第4题单选题(5分)（）isthemainpathogeninthesterilizationofcans.AClostridiumbotulinumBStaphylococcus

aureus

CLactobacillusDBacillussubtilis第5题单选题(5分)（）cancausefoodbulgingspoilage.AP.acidobacteriumBhighatmosphericpressureCnotopgapDflatsour(ing)第6题单选题(5分)Theprocessofdryingfruitsandvegetablesisaprocessthat().AsterilizationandenzymekillingBsterilization,noenzymekillingCenzymekilling,nosterilizationDnosterilizationandnoenzymekilling第7题判断题(5分)Cannedstoragemeansthatallpathogenicbacteriaintheproducthavebeenkilled,thesurvivalprobabilityofheat-resistantnon-pathogenicbacteriahasreachedthespecifiedrequirements,anditisimpossibletogrowandreproduceinanormalsalesperiodundersealedandintactconditions.第8题判断题(5分)Semi-fluidfoodintheprocessofsterilizationandheatingarebyconduction.第9题判断题(5分)Inordertopreventthedeformationofthecan,acertainamountofbackpressureshouldbeaddedthroughoutthesterilizationprocess.第10题判断题(5分)Duringthesterilizationprocess,thepressureinsidethecannedpotatokeepsrisingbecauseoftheairescapingfromthetissue.第11题判断题(5分)Thereisnomicrobialresidueaftercannedsterilization.第12题多选题(9分)Commonheattransfermethodsofcannedfoodinclude().AconductionBconvectionCradiationDcontact正确答案：ABC第13题多选题(9分)ThegeneralstepstodeterminethesterilizationFvalueare:().AidentifythebacteriathatoftenspoilthecannedfoodBheatresistanceofthebacteria(Zvalue,Dvalue)CthesafeFvaluewascalculatedaccordingtotheformulaDthechangeofthetemperatureinthecenterofthecanduringtheactualsterilizationprocesswasmeasured.Accordingtothecentertemperature,theactualsterilizationFvaluewascalculatedandcomparedwiththesafeFvalue正确答案：ABCD第14题多选题(9分)Whatarethefactorsthataffectthevacuumdegreeofcans?AexhausttimeandtemperatureBvacuumofsealedroomCsealingtemperature,externalpressureandtemperaturechangeDtop

gap

of

cans正确答案：ABCD第15题多选题(9分)Whatfactorswillaffecttheheattransfereffectofcannedfood?()AphysicalpropertiesoffoodincansBinitial

temperatureCcannedcontainerDsterilizationpot正确答案：ABCD第16题多选题(9分)Themethodofcansexhaustinclude().Ahot-filled

BthermalexhaustCsteamexhaustDvacuumexhaust正确答案：ABCDChapter9exercises第1题单选题(7分)Immunoassayisamethodbasedontheprincipleof（）specificbindingreaction.ADNAandRNABantigens