糖代谢途径全解#知识材料

1、AcCOA：乙酰辅酶A；CIT：柠檬酸；DHAP：磷酸二羟丙酮；E4P：4-磷酸赤藓糖；F6P：6-磷酸果糖；F1,6P：1,6-二磷酸果糖；FUM：延胡索酸；GA3P：3-磷酸甘油醛；G3P：3-磷酸甘油；G6P：6-磷酸葡萄糖；G6PA：6-磷酸葡萄糖酸；-KG：酮戊二酸；LAC：乳酸；MAL：苹果酸；OAA：草酰乙酸；PEP：磷酸烯醇式丙酮酸；3PG：3-磷酸甘油酸；PYR：丙酮酸；Ru5P：5-磷酸核酮糖；R5P：5-磷酸核桃；SUC：琥珀酸；SUCCoA：琥珀酰辅酶A；X5P：5-磷酸木酮糖. 胞内的代谢反应方程糖酵解途径 Glycolysis pathway rl GLU+ATPG

2、6P+ADPr2 G6PF6Pr3 F6P+ATPDHAP+G3AP+ADPr4 DHAP+NADHG3P+NADr5 G3P+ADPGLY+ATPr6 GA3P+NAD+ADP 3PG+NADH+ATPr7 3PGPEPr8 PEP+ADPPYR+ATP三羧酸循环 Tricarboxylic acid cycle r11 PYR+CoA+NADAcCoA+NADH+CO2r12 PYR+ATP+CO2OAA+ADPr14 OAA+AcCoAICI+CoAr15 ICI+NAD-KG+NADH+CO2r16 -KG+CoA+NADSucCoA+NADH+CO2r17 SucCoA+ADP+Pi

3、SUC+ATP+CoAr18 SUC+FADFUM+FADH2r19 FUMMALr20 MAL+NADOAA+NADH磷酸戊糖途径 Plentose phosphate pathwayr21 G6P+NADPG6PA+NADPHr22 G6PA+NADPRu5P+NADPH+CO2r23 Ru5P0.67X5Pr24 Ru5P0.33R5P r25 X5P+R5PF6P+E4Pr26 X5P+E4PF6P+GA3P副产物形成 By-products synthesis r9 PYR+NADHLactate+NADr10 PYR+NADHEthanol+CO2+NADr13 PYRPyrExt

4、氧化磷酸化 Oxidative phosphorylationNADH+0.5O2+(P/O)ADPNAD+(P/O)ATP FADH2+0.5O2+0.5(P/O)ADP0.5(P/O)ATP+FAD用于维持的ATP消耗 ATP consumption for maintenance ATPADP+Pi生物量形成 Biomass formation0.067G6P+0.064R5P+0.009GA3P+0.065PG+0.050PEP+0.176Pyr+0.095OAA+0.102KG+0.249AcCoA+1.142NADPH+0.157NAD+3.82ATPBiomass+1.142NADP+0.157NADH+0.127CO2+3.8-2ADP图4 补料发酵过程中胞浆3-磷酸甘油脱氢酶、6-磷酸葡萄糖脱氢酶、丙酮酸激酶酶活的时序变化Fig. 4 Time courses of the activity of ctGPD, G6PDH and PYK in fed-batch culturectGPD：胞浆3-磷酸甘油脱氢酶；G6PDH：6-磷酸葡萄糖脱氢酶；PYK：丙酮酸激酶ctGPD: cytoplasmic glycerol 3-phosphate dehydro