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1HPLC法测定芦丁中芦丁和槲皮素的含量A1A0A3A4A2A4A2A5A6HPLCA7A8A9A10A11A9A10A12A13A14A15A16A17A18A19A20A21A20A21A22A23ZORBOXSBC18A24A25A26A27A28A29A30A31A32A33A34A35A36A3745A3850A385A39A27A28A4010MLMIN1,A41A7A42A43256NMA19A44A45A9A10A4660140GML1A47A48A49A50A51A52A53A54A55A56A57A58A52R100000A52A59A60A61A622456GML1A47A48A49A50A51,A53A54A55A56A57A58A52R099998A63A64A65A66A67A68A69A70A71A72993A52RSDA7206A52A59A60A61A66A67A68A69A70A71A72996A52RSDA7204A63A73A74A75A76A77A78A79A80A52A81A82A54A57A83A58A52A84A85A86A87A88A89A90A91A92A93A94A95A96A97A98A99A100A97HPLCDETERMINAITIONOFRUTINANDITSQUERMINATIONBYHPLCABSTRACTOBJECTIVEAHPLCMETHODFORTHEDETERMINATIONOFRUTINANDITSQUERMINATIONWASESTABLISHEDMETHODSAZORBOXSBC18COLUMNWASUSEDA101WITHTHEMOBILEPHASEOFWATERMETHANOLVINEGAR45A9450A945A101ATTHEFLOWRATEOF10MLMIN1A101THEDETECTIONWAVELENGTHWAS256NMRESULTSTHELINEARRANGEOFRUTINR100000WAS60140UGML1A101ANDQUERMINATIONR099998WAS2456A102GML1A101THEAVERAGERECOVERYOFRUTINWAS993A101RSD06A101ANDQUERMINATIONWAS996A101RSD04A103CONCLUSIONITWASACCURATEANDRAPIDKEYWORDSA94RUTINA97QUERMINATIONA97HPLCA95A96A104A105A106A107A108A109A106A107A108A110A111A112A113A114A115A116A117A118A119A120A121A122A123A124A125A122A126A127A128A129A123A130A131A132A133A134A135A136A137A138A139A140A141A142A143A144A145A146A147A148A149A147A142A150A151A132A152A153A151A154A155A156A157A140A158A159A142A160A161A156A162A132A163A164A165A133A166A167A168A169A170A171A172A173A142A152A153A174A175A132A176A177A178A179A180A181A182A183A184A185A151A186A187A188A189A189A190A191A192A172A173A193A194A165A151A195A196A197A191A192A198A199A200A142A193A194A165A201A191A202A203A165A204A150A205A206A165A207A208A198A199A200A209A191A210A194A165A211A151HPLCA191A204A150A212A213A165A214A215A184A216A1321A217A218A219A220A221A218A219A220A22111A222A223A224A225A226A227A228A229A222A230AGILENT1100A231A232A233A234A235A236A232A237A238A223A239HPCHEMSTATIONA240A2412A242A243A244A245AGILENT1100A246A247A248A249A250A251A252A245AGILENT1100A246A247A253A254A255A245AGILENT1100A246A247A172A0A1A1512A175A2A26A3A30TEDIACOMPANYA245INCUSAA32A24A4A5A6A245A28A3A80A28A7A8A24A9A10A6A72A11A12A30A177A13A71040609A80040908A80041107A32A245A179A14A16A17A18A19A20A240A242A21A245A11A12A22A23A25A27A29A31A33A22A23A25A30A8A24A50A34A2A25A35A36A37A25A38A39A40A245A41A42A8A241000A322A43A44A45A46A47A44A45A46A4721A48A49A51A52A48A49A53A54AGILENTZORBOXSBC1846MMA194150MMA555A56A57A58A59A60A61A62A63A64A65A6645A6750A675A68A69A70A7335A195A69A57A7410MLMIN1A69A75A76A77A78256NMA69A168A79A8120LA187A196211A197A82A196222A197A198A83A84A85A86A87A88A187A89A90A201A91A89A92A93A69A203A168A7920LA204A94A95A96A97A66A971A68A69A207A208A98A99A100A101A102A103A1042A10556MINA106A107A108A1095A105831MINA106A211A100A110A111A102148A112A213A113A114A115A116A117A118A1192000A120A103A1048700A106A107A108A1094800A12122A169A122A123A124A125A126A127221A186A128A122A123A124A125A126A127A129A130120A131A220A132A133A134A135A125A103A104A186A128A1222000MGA136A107A108A109A186A128A12280MGA106A224A137A138100MLA139A140A141A106A142A143A144A145A146A147A123A148A106A151A149A150A152A103A10401MGML1A106A107A108A1094GML1A153A154A155A156A157A158A159A160222A161A162A157A158A159A163A164A165A231A166A167A170A171A157A234A173A174A176A178A180A181A182200MGA183A184A185100MLA236A173A225A188A184A189A190A191A176A192A193A158A199A184A200A202A205A206A209A210A184A238A212A160A231A166A173A170A214A215A158A1595MLA185100MLA236A173A225A188A184A189A190A191A176A202A205A206A209A210A184A238A212A184A153A154A161A162A157A158A159A16023A216A217A218A219A221A222A223A226A227A228A229A230A232A233A2352000MGA237A239A240A241A232A233A23580MGA237A244A242A243A245A246A247A248A249A237A223A226A250A251A252A229A2302MGML1A253A239A240A241008MGML1A254A248A255A237A245A0A1A2A248A255A3A4A255A223A226A5A250A251A252A229A230006A80008A80010A80012A80014MGML1A253A252A239A240A24124A8032A8040A8048A8056GML1A232A233A235A248A255A15A223A226A142A620LA7A8A237A245A9A10A11A3A12A13A14A237A186A16A17A18A19A20A21A22A23A24A190A23A58A25A26A27A28A29A30A31A32A33A34A35A23A58A25A26A36A37A38A39A40A20A71Y06161919236951833XA27R100000Y02390663251588835XA27R0999983A26A43A41A42A39A40A20A29A3060140GML1A27A32A33A342456GML1A4424A168A45A46A47A19A48A49A71A86A196221A197A198A50A35A186A16A17A51A52A27A53A240A54A55A56A57A59A168A456A60A27A29A30A61A62A63A35RSDA2001A27A32A33A34A61A62A63A35RSDA2008A4425A64A65A43A48A49A86A29A30A66040609A67A53222A198A50A68A69A190A70A72A48A17A51A525A73A27A53A45A17A169A74A75A169A74A27A76A77A68A78A79A81A20992N5A27A82A83A84A75A64A65A43A85A4426A72A48A17A51A52A87A74A43A48A49A71A86A84A17A53A79A81A169A74A37A75A88A89A90A55A56A39A40A91A2240A922A924A926A928A9210A9212HA169A74A76A77A27A68A78A79A81A20A29A30993A27RSDA2006A27A32A33A34032A27RSDA2004A44A93A83A72A48A17A51A52A15812A94A95A126A96A97A98A87A74A35A4427A36A99A100A48A49A71A39A40A46A47A101A86A29A30A31A32A33A34A186A16A17A102A81A27A103A104A105A106A107A108A51A109A110A111A112A113A23A114A81A18A19A3580A92100A92120A203A115A73A44A53A79A81A169A74A37A75A169A74A36A99A100A44A169A28A29A30A35A68A78A36A99A100A39A40A20993A27RSDA2003A116A32A33A34A68A78A36A99A100996A27RSDA2006A27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