Takara-双酶切buffer用表

1、精选优质文档-倾情为你奉上EnzymeAcc IBamH IBgl IICla IEcoR IEcoR VHinc IIHind IIIKpn INco INde INot IPst IPvu ISac ISal ISma ISpe ISph IXba IXho ISupplied Buffer10 ×M10 ×K10 ×H10 ×M10 ×H10 ×H10 ×M10 ×M10 ×L10 ×K+BSA10 ×H10 ×H+BSA +Triton10 ×H10 

2、15;K+BSA10 ×L10 ×H10 ×T+BSA10 ×M10 ×H10 ×M+BSA10 ×HAcc I-0.5 × K1 ×T1 ×M1 ×M0.5 × K1 ×M1 ×M1 ×M1 ×M+BSA1 ×T0.5 × K+BSA1 ×M0.5 × K1 ×M1.5 × T1 ×T+BSA1 ×M0.5 × K1 ×M1 ×

3、;MBamH I0.5 × K-1 ×K1 ×K1 ×K1 ×K0.5 × K1 ×K0.5 × K1 ×K+BSA1 ×K0.5 × K+BSA1 ×K1 ×K0.5 × K1.5 × T0.5 × T+BSA1 × K1 × K0.5 × K1 × KBgl II1 × T1 × K-1 × H1 × H1 × H2 × K1 

4、15; K1 × T1 × K+BSA1 × H1 × H+BSA1 × H1 × K0.5 × K1 × H1 × T+BSA1 × H1 × H2 × T1 × HCla I1 × M1 × K1 × H-1 × H1 × H1 × M1 × M1 × M1 × K+BSA1 × H1 × H+BSA1 × H1 × K1 ×

5、; M1 × H1 × T+BSA1 × M1 × H1 × M1 × HEcoR I1 × M1 × K1 × H1 × H-1 × H1 × M1 × M1 × M1 × K+BSA1 × H1 × H+BSA1 × H1 × K1 × M1 × H1 × T+BSA1 × H1 × H1 × M1 × HEcoR V0.5 

6、5; K1 × K1 × H1 × H1 × H-2 × T1 × K0.5 × K1 × K+BSA1 × H1 × H+BSA1 × H1 × K0.5 × K1 × H0.5 × K+BSA1 × H1 × H2 × T1 × HHinc II1 × M0.5 × K2 × K1 × M1 × M2 × T-1 × M1 ×

7、; M1 × M+BSA1 × T0.5 × K+BSA1 × M0.5 × K1 × M1.5 × K1 × T+BSA1 × M2 × T1 × M1 × MHind III1 × M1 × K1 × K1 × M1 × M1 × K1 × M-1 × M1 × K+BSA1 × K0.5 × K+BSA1 × M1 × K1 × M

8、1.5 × K1 × T+BSA1 × M1 × K1 × M1 × MKpn I1 × M0.5 × K1 × T1 × M1 × M0.5 × K1 × M1 × M-0.5 × K+BSA1 × T0.5 × K+BSA1 × M0.5 × K1 × L1.5 × T+BSA1 × T+BSA1 × M0.5 × K1 × M1 ×

9、 MNco I1 × M+BSA1 × K+BSA1 × K+BSA1 × K+BSA1 × K+BSA1 × K+BSA1 × M+BSA1 × K+BSA0.5 × K+BSA-1 × K+BSA0.5 × K+BSA1 × K+BSA1 × K+BSA0.5 × K+BSA1.5 × T+BSA1 × T+BSA1 × K+BSA1 × K+BSA1 × M+BSA1 × K+BSANde

10、I1 × T1 × K1 × H1 × H1 × H1 × H1 × T1 × K1 × T1 × K+BSA-1 × H+BSA1 × H1 × K1 × T1 × H1 × T+BSA1 × H1 × H1 × T1 × HNot I0.5 × K+BSA0.5 × K+BSA1 × H+BSA1 × H+BSA1 × H+BSA1 ×

11、; H+BSA0.5 × K+BSA0.5 × K+BSA0.5 × K+BSA0.5 × K+BSA1 × H+BSA-1 × H+BSA2 × K+BSA0.5 × K+BSA1 × H+BSA0.5 × T+BSA1 × H+BSA1 × H+BSA0.5 × K+BSA1 × H+BSAPst I1 × M1 × K1 × H1 × H1 × H1 × H1 × M1 ×

12、; M1 × M1 × K+BSA1 × H1 × H+BSA-1 × K1 × M1 × H0.5 × T+BSA1 × H1 × H1 × M1 × HPvu I0.5 × K1 × K1 × K1 × K1 × K1 × K0.5 × K1 × K0.5 × K1 × K+BSA1 × K2 × K+BSA1 × K-0.5 × K

13、1.5 × K+BSA1 × K+BSA1 × K1 × K0.5 × K1 × KSac I1 × M0.5 × K0.5 × K1 × M1 × M0.5 × K1 × M1 × M1 × L0.5 × K+BSA1 × T0.5 × K+BSA1 × M0.5 × K-1.5 × T+BSA1 × T+BSA1 × M0.5 × K1 × M

14、1 × MSal I1.5 × T1.5 × T1 × H1 × H1 × H1 × H1.5 × K1.5 × K1.5 × T+BSA1.5 × T+BSA1 × H1 × H+BSA1 × H1.5 × K+BSA1.5 × T+BSA-1.5 × T+BSA1 × H1 × H1.5 × T1 × HSma I1 × T+BSA0.5 × T+BSA1 

15、15; T+BSA1 × T+BSA1 × T+BSA0.5 × K+BSA1 × T+BSA1 × T+BSA1 × T+BSA1 × T+BSA1 × T+BSA0.5 × T+BSA0.5 × T+BSA1 × K+BSA1 × T+BSA1.5 × T+BSA-1 × T+BSA0.5 × T+BSA1 × T+BSA1 × T+BSASpe I1 × M1 × K1 × H1 ×

16、; M1 × H1 × H1 × M1 × M1 × M1 × K+BSA1 × H1 × H+BSA1 × H1 × K1 × M1 × H1 × T+BSA-1 × H1 × M1 × HSph I0.5 × K1 × K1 × H1 × H1 × H1 × H2 × T1 × K0.5 × K1 × K+BSA1 × H1

17、 × H+BSA1 × H1 × K0.5 × K1 × H0.5 × T+BSA1 × H-2 × T1 × HXba I1 × M0.5 × K2 × T1 × M1 × M2 × T1 × M1 × M1 × M1 × M+BSA1 × T0.5 × K+BSA1 × M0.5 × K1 × M1.5 × T1 × T+BSA1 &

18、#215; M2 × T-1 × MXho I1 × M1 × K1 × H1 × H1 × H1 × H1 × M1 × M1 × M1 × K+BSA1 × H1 × H+BSA1 × H1 × K1 × M1 × H1 × T+BSA1 × H1 × H1 × M-Note: 1) It is confirmed that 10 units of each enzyme

19、completely digest 1 µg of DNA at 37°C in one hour in 50 µl reaction mixture. 2) The concentration of Glycerol should be less than 10% to minimize star activity. 3) DNA may not be digested completely, when recognition sequences of two enzymes are close each other, or when DNA takes hig

20、h-structure conformation.Relative activity for each reaction buffer: supplied, activity measured buffer: recommended bufferRestriction enzymeRelative activities (%)LMHKT+BSABasal*Aat II<20<20<20<20100120Acc I20100<20(<20)16080Acc II(260)100<2020200160Acc III(<20)(<20)20(80

21、)(<20)100Afa I60604060100100Afl II2080*<20<20140120Alu I100100<2040200120Aor13H I<2020<2080*80100Aor51H I80100<2020120120Apa I100<20<20<20<20120ApaL I10020<20<20120120Ava I(<20)1002040100120Ava II80100<2020100100Bal I2020<20<2040100BamH I(<20)<

22、;2040100(<20)80Ban II(120)(120)10080(100)100Bbe I<20<20<20<20<20100Bcn I<2020406060100Bgl I<20<202040<20100Bgl II<2020100(100)(60)100Bln I<20204010020120BmeT110 I<20<2020100<20140BmgT120 I<20<2010040<20240Bpu1102 I<20<20<204060100BspT104

23、 I10060<20<20100120BspT107 I<20208010020100Bsp1286 I10020<20<2060100Bsp1407 I20602020100100BssH II1001006020140100BstP I(<20)(60)100(100)(100)100BstX I<2040100<20<20120Bst1107 I(<20)6010010040100Cfr10 I(<20)(<20)(<20)40(20)100Cla I4010012010060100Cpo I<20<

24、;2080100<20100Dra I100100601008080Eae I60100<20<20120160Eam1105 I(<20)(40)2040(40)100EcoO65 I(20)(60)60*4040100EcoO109 I10060<20<20100160EcoR I(20)(100)100(120)(80)120EcoR V(<20)(40)100(120)(40)100EcoT14 I(<20)(40)100120(60)100EcoT22 I<2020100(140)(20)120Eco52 I<20<2

25、0<20<20<20100Eco81 I<20100<20<20100160Fba I(<20)(<20)(80)100(20)100Fok I(20)(60)<20<20(200)100Hae II80100<2080140100Hae III6010010060100100Hap II10060<20<2010080Hha I80100100120120100Hinc II20100204010080Hind III(60)100<20200(100)80Hinf I8010010016060100Hin1

26、 I4080*<202060160Hpa I<20(40)20100(80)100Kpn I10060<20<20(100)80Mbo I20406010040100Mbo II10060<20<2060100Mfl I10080<20<2080100Mlu I6060100(100)60100Msp I8080<2010010080Mun I(200)100*<20<20160100Mva I(<20)(40)80100(20)120Nae I100<20<20<20100120Nco I(40)(60

27、)2060*(60)160Nde I<204010010080100Nhe I(120)100<20<20(160)100Not I(<20)(<20)20*<20(<20)100Nru I0<202020<20100Nsb I4020<2060100100PmaC I10080<20<20100100PshA I2040<2010060160PshB I(20)(40)204040100Psp1406 I2060<20<20100100Pst I(<20)(60)10080(20)80Pvu I(

28、<20)(20)(40)80*(40)120Pvu II(80)10040<20(40)100Sac I10060<20<208080Sac II4020<20<2010040Sal I<20<20100(20)<20120Sau 3A I(60)80100<20(80)100Sca I(<20)(<20)100(60)(<20)100Sfi I(40)100<20<20100100Sma I<20<20<20<20100100Smi I<10<2010040<1

29、0100SnaB I(20)(40)<20<20(40)100Spe I(80)10080100(80)100Sph I(20)(40)100120(20)100Sse8387 I(120)60*<20<20(60)100Ssp I(<20)(60)40(100)(80)100Stu I601006080140100Taq I4080606080100Tth111 I(20)8040100(80)120Van91 I<20(20)60100(60)100VpaK11B I<20<2060(40)<20100Xba I<2080*20&

30、lt;20120120Xho I<206010016060100Xsp I<2060<20100160100Compositions of universal buffer1.10× L100 mMTris-HCl (pH7.5)100 mMMgCl210 mMDithiothreitol2.10× M100 mMTris-HCl (pH7.5)100 mMMgCl210 mMDithiothreitol500 mMNaCl3.10× H500 mMTris-HCl (pH7.5)100 mMMgCl210 mMDithiothreitol1,000 mMNaCl4.