2-4-D-ammonium-2-4-Dichlorophenoxyacetic-acid-ammonium-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors • ScreeningLibraries • Proteinswww.MedChemE2,4-DammoniumCat.No.:HY-W547534CASNo.:2307-55-3Synonyms:2,4-Dichlorophenoxyaceticacidammonium分子式:C₈H₉Cl₂NO₃分子量:238.07作用靶点:Herbicide;DNA/RNASynthesis;Apoptosis作用通路:MetabolicEnzyme/Protease;CellCycle/DNADamage;Apoptosis储存方式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY生物活性2,4-D(2,4-Dichlorophenoxyaceticacid)ammonium是一种可用于阔叶杂草防治的选择性除草剂。2,4-Dammonium可诱导细胞凋亡(apoptosis)。2,4-Dammonium抑制DNA和蛋白质的合成，从而阻止植物的正常生长发育。体外研究2,4-D(25-200μM,72h)ammoniuminhibitsthecellviabilityofA549andWI38withIC50valuesof126µMand115µM,respectively.Inducesapoptosisbyinfluencingthecellcycle[2].CellViabilityAssayWesternBlotAnalysisCellProliferationAssayApoptosisAnalysisCellCytotoxicityAssayCellCycleAnalysisRT-PCRCellAutophagyAssayImmunofluorescenceCellDifferentiationAssayCellInvasionAssayCellMigrationAssayRealTimeqPCRELISAAssay[2]CellLine:A549,W138Concentration:25,50,75,100,150,200μMIncubationTime:72hResult:Inhibitedcellviabilityinadose-dependentmanner.CellCycleAnalysis[2]CellLine:A5491/3 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemEConcentration:50,100,150μMIncubationTime:72hResult:TheG0/G1populationdecreasedtoabout57%,43%and31%,respectively.CellViabilityAssayWesternBlotAnalysisCellProliferationAssayApoptosisAnalysisCellCytotoxicityAssayCellCycleAnalysisRT-PCRCellAutophagyAssayImmunofluorescenceCellDifferentiationAssayCellInvasionAssayCellMigrationAssayRealTimeqPCRELISAAssay[2]CellLine:A549,W138Concentration:100,150μMIncubationTime:72hResult:Theearlyapoptoticpopulationincreasedto44%inA549cellsand57%inWI38cells,andthelateapoptoticpopulationincreasedto18%inA549cellsand10%inWI38cells.WesternBlotAnalysis[2]CellLine:A549,W138Concentration:100,150μMIncubationTime:72hResult:Up-regulatedp53andBaxproteins,down-regulatedBcl-2andprocaspase3.体内研究2,4-D(100or200mg/kg,gavagefor30days)ammoniumhastoxiceffectsonthereproductivesystemofmalerats[3].AnimalModel:AdultWistarmalerats[3]Dosage:100or200mg/kgAdministration:i.g.Result:Decreasedthetestis,seminalvesiclesandprostaterelativeweight.Decreasedthenumberofspermatozoaandspermmotility.IncreasedthelevelofFSHandLH.客户使用本产品发表的科研文献• AnalChem.2025Jun3;97(21):11099-11109.2/3 MasterofBioactiveMolecules—您身边的抑制剂大师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCESGermaineKJ,etal.Bacterialendophyte-enhancedphytoremediationoftheorganochlorineherbicide2,4-dichlorophenoxyaceticacid.FEMSMicrobiolEcol.2006Aug;57(2):302-10.GanguliA,etal.2,4-Dichlorophenoxyaceticacidinducedtoxicityinlungcellsbydisruptionofthetubulin-microtubulenetwork.ToxicologyResearch,2014,3(2):118-130.MarouaniN,etal.Effectsoforaladministrationof2,4-dichlorophenoxyaceticacid(2,4-D)onreproductiveparametersinmaleWistarrats.EnvironSciPollutResInt.2017Jan;24(1):519-526.McePdfHeightCaution:Producthasnotbeenfullyvalida