OAC2-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEOAC2Cat. No.: HY-12884CAS No.: 6019-39-2分式: CHNO分量: 236.27作靶点: Oct3/4作通路: Stem Cell/Wnt储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (423.24 mM)Ethanol : 25 mg/mL (105.81 mM; Ne

2、ed ultrasonic)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (10.58 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)1/2 Master of Small Molecules 您边的抑制剂师www.MedChemESolubility: 2.5 mg/mL (10.58 mM); Suspended solution; Need ultrasonic3. 请依序添加每种溶剂： 10% DMSO 90% corn

3、oilSolubility: 2.5 mg/mL (10.58 mM); Clear solution4. 请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (10.58 mM); Clear solution5. 请依序添加每种溶剂： 10% EtOH 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (10.58 mM); Suspended solution; Need ultrasonic6. 请依序添加每种溶剂： 10% EtOH 90%

4、corn oilSolubility: 2.5 mg/mL (10.58 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 OAC2Oct4激活化合物，其通过Oct4 因启动激活表达。体外研究 Octamer-binding transcription factor 4 (Oct4) is a master regulator of the induction and maintenance ofcellular pluripotency, and has crucial roles in early stages of differentiation. It

5、 is the only factor that cannot besubstituted by other members of the same protein family to induce pluripotency 1. Oct4 has been shown tobe an essential regulator of embryonic stem cell (ESC) pluripotency and key to the reprogramming process.OAC2 is a structural analog of OAC1. OAC2 activates both

6、Oct4 and Nanog reporters to a similar extent asOAC1. OAC1 and its two structural analogs OAC2 and OAC3 enhances reprogramming efficiency fourfold,up to as high as 2.75%, and accelerates the appearance of iPSC colonies 3 to 4 d when used in combinationwith the four reprogramming factors, Oct4, Sox2,

7、Klf4, and c-Myc 2.PROTOCOLCell Assay 2 The Oct4-luc or Nanog-luc cells are treated with compound OAC1 or its structural analogs OAC2, OAC3 at 1M concentration or at indicated concentrations. Other compounds used include 2 M BIO, 2 M BIX, 2 M5-azacytidine, 25 g/mL Vitamin C, 10 nM Am580, 5 M tranylcy

8、promine, and 0.5 mM valporic acid.Luciferase reporter assays are performed 24 h after compound treatment or at indicated time points. ForTopflash reporter assays, 0.2 g -cateninresponsive Topflash reporter gene plasmid is introduced into CV1cells using trasfection. Compounds are added 6 h after tran

9、sfection. Luciferase activity is measured 48 hafter compound treatment using the Glo Luciferase Assay System 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Okuyama T, et al. Structural and mechanistic insights into nuclear transport and

10、delivery of the critical pluripotency factor Oct4 to DNA. JBiomol Struct Dyn. 2017 Feb 6:1-502. Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A. 2012 Dec18;109(51):20853-8.McePdfHeight2/2 Master of Small Molecules 您边的抑制剂师www.MedChemECaution: Product has not been fully vali